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(Source: D. Frost and D. Sepich [from Zebrafish Book 5th Edition|http://zebrafish.org/zirc/orders/buyBookQ.php?item=Book&id=book&detail=The%20Zebrafish%20Book])
# Place embryos into high calcium Ringer. Cut or pull into pieces using sharpened forceps or needles.
# Rinse by dipping into growth medium twice.
# Plate onto tissue culture dish in a single drop of medium with 5% serum (to help attachment).
# Incubate 4 hours at 28.5°C.
# Gently fill the dish with growth medium without serum. |
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