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Works well for a range of 3-15 micron thick sections on both embryonic and adult tissue
Cryoprotection
Fix and rinse the tissue. After rinsing the tissue at room temperature in 0.1 M phosphate buffer with 5% sucrose, cryoprotect with increasing concentrations of sucrose by mixing 5% and 20% (0.6 M) sucrose on phosphate buffer in ratios of 2:1, 1:1, 1:2. Infiltrate the tissue for ½ hour in each sucrose mixture at room temperature, and then cryoprotect with 20% sucrose in phosphate at 4° C overnight. All fixation, rinses and infiltrations are done with gentle rotation.
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