...
4. Can look at embryos on an epifluorescence or confocal microscope at this point or continue with detection of next hapten. Wash longer if high background.
Second color reaction:
Begin protocol for 10 Antibody labeling for the 2nd probe:
...
...
4. Can look at embryos on an epifluorescence or confocal microscope at this point or continue with detection of next hapten. Wash longer if high background.
Second color reaction:
Begin protocol for 10 Antibody labeling for the 2nd probe:
...