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Instead, why not just use pCSDest (#201 from Lawson lab:http://lawsonlab.umassmed.edu/GWDestplasmids.htm) to make a pCS2-like plasmid containing your gene of interest? Then you can use T7 to make antisense probe.
AUGUST 31, 2007 6:37 AMSeokAM
Seok-Yong said...
Many thanks for swift response. As you suggested, I will generate another construct using pCSDest. Thanks again. SY
AUGUST 31, 2007 1:16 PMSeokPM
Seok-Yong said...
Hi,
Does anyone have experience with generating DIG-labeled RNA from a gateway vector?
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