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1.
...
Chill
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200
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3d
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embryos
...
after
...
removing
...
from
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chorions.
...
2.
...
Rinse
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in
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0.5%
...
chilled
...
bleach
...
for
...
2
...
minutes
...
and
...
then
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in
...
zero
...
calcium
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Ringer
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for
...
2
...
minutes.
...
3.
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Transfer
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to
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a
...
Dounce
...
homogenizer
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with
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a
...
mini-mum
...
of
...
liquid
...
and
...
homogenize
...
well.
...
4.
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Resuspend
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in
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1
...
ml
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L-15
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supplemented
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with
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0.3
...
mg/ml
...
glutamine,
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50
...
U/ml
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penicillin,
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0.05
...
mg/ml
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streptomycin
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and
...
0.8
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mM
...
CaCl2.
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5.
...
Store
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at
...
-20°C.
...
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