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The Tol2kit is described in a paper in Developmental Dynamics; complete documentation including protocols and lists of constructs can be found at the Chien lab's Tol2kit documentation site. A related set of constructs based on 2-insert Multisite Gateway are described at the Lawson lab Gateway site.
This wiki page is intended for discussion and questions about the Tol2kit. The Chien lab will monitor posted comments and try to respond quickly. A public blog was originally used for this purpose; we are currently in the process of transferring questions and comments from the blog to this page.
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