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7. Store slides in a box in a -20o C . Freezer freezer for up to one year.
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Day 1
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Day 5
47. Wash slides for 4 times X in TNT at room temperature for over 1 hour total.
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52. Wash 2 X 5 minutes in TNT.
53. Quench development reaction by washing for 15 minutes in 2% H2O2 in TNT.
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Mix well, and heat @ 68˚ C for one hour to get powder into solution.
Aliqoute Aliqout and store at -20˚C, and once thawed, never refreeze.
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