(Source: T. Montgomery from Zebrafish Book 5th Edition)
Siphon (cleaning by "vacuuming") the bottoms of all tanks at leastonce per week. Use siphon tips with a length that corresponds to the size of the tank: tips for 5-gallon tanks are 12" long, the 10-gallon tips are 18" long and the 30-gallon tips are 26" long. Each tip is made of ½" I.D. x 1/16" Wall Plexiglas tubing (Port Plastics, Portland, Oregon). Cover one end with a one inch tip of 3/8" I.D. x 3/32" Wall Amper Latex tubing at one end (VWR Scientific Co.). (See Embryo Collection, for a diagram.) The siphon tips, which are attached to the hoses, are the only parts of this apparatus that can be immersed in the tank. Use each tip in only one tank and then sterilize it by soaking in a bleach solution (solution is 20 parts water to 1 part bleach) followed by a rinse in a pipette washer.
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