(Source: J. Eisen from Zebrafish Book 5th Edition)
In principle, almost any cell in the zebrafish embryo that can be visualized (e.g. with Nomarski DIC optics) can be ablated by laser‑irradiation. The power output of the laser and the type of optical system used to focus the beam onto the cell will determine whether, in practice, a particular cell can be ablated. The following protocol has been used successfully to ablate single identified neurons in the spinal cord, individual floor plate, muscle, notochord, and neural crest cells, and entire ganglia.
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Once the cell of interest has been ablated, the embryo should be removed from the agar or the bridged coverslip and placed in a dish of Embryo Medium to continue development.
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