(Source: J. Eisen from Zebrafish Book 5th Edition)
Preparation of micropipettes
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5. Mount a labeled donor and an unlabeled host embryo side by side. Since many cells can be taken from one labeled embryo, it is often expedient to mount a single donor embryo and 3-4 host embryos in the same chamber.
Figure 14. Transplantation slide with agar-mounted embryos.
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A word of caution, anesthetics such as tricaine (ethyl-m-aminobenzoate methanesulfonate) will kill single cells during the transplantation procedure. Therefore, if you need to transplant cells between embryos that are twitching, rather than using an anesthetic you should use a nonmotile mutant.
Dismounting embryos
Remove embryos from the agar by gently separating the sides of the agar where it has been cut to expose a portion of the embryo (see section on Agar Mounting, page 4.5). Move the embryos to fresh Ringer containing penicillin and streptomycin (1% v/v penicillin/streptomycin solution containing 5,000 units penicillin and 5 mg streptomycin per ml in 0.9% sodium chloride in Ringer). It is essential that the embryos be maintained in Ringer until the hole in the skin heals, or else they will fall apart.