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29. Replace amplification diluent with 100-200 µl of 1:50 Tyr-Fluorescein. Let reaction develop for 45 minutes in the dark. Don't Don’t exceed one hour. All steps from here on out are done in the dark.
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Fluorescein Labeling Mix: Roche Cat# 11685619910.
20X NTP mix: 100mM NTP's NTP’s obtained from Amersham Bioscience (Cat# 27202501).
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( Sigma: Cat#R-6750-1G) (Ribonucleic acid from baker's baker’s yeast)
Dissolve in DEPC treated or RNAase free H2O 10 mg/ml final concentration.
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