Prepare 100xE2A by dissolving the following ingredients in a final volume of 1600 ml:
140.0g NaCl
6.0g KCl
19.2g MgSO4
3.3g KH2PO4
1.1g Na2HPO4

• add Millipore water to 1600 ml
• shake and stir to dissolve the reagents
• autoclave
• stir O/N to dissolve any precipitation that has formed during autoclaving
• store at 40C