PREHYB AND HYBRIDIZATION MIX (HM)
Formamide 50 65%
5 x SSC
Tween20 0.1%
Citric acid to pH 6.0 (460 μl of 1M for 50 ml)
Heparin 50 μg/ml
tRNA 500 μg/ml
Formamide 50 65%
5 x SSC
Tween20 0.1%
Citric acid to pH 6.0 (460 μl of 1M for 50 ml)
Heparin 50 μg/ml
tRNA 500 μg/ml