Light sheet imaging (e.g. SPIM)

Owned by jhuisken
Last updated: May 11, 2011Version comment
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Light sheet microscopy, most commonly refered to as Selective Plane Illumination Microscopy (SPIM), has been shown to perform extremely well in large, living embryos such a zebrafish embryos and larvae. Here is a list of references that demonstrate the use of SPIM in zebrafish:

  • Arrenberg et al. Optogenetic control of cardiac function. Science (2010) vol. 330 (6006) pp. 971-4
  • Liu et al. A dual role for ErbB2 signaling in cardiac trabeculation. Development (2010) vol. 137 (22) pp. 3867-75
  • Bagnat et al. Cse1l is a negative regulator of CFTR-dependent fluid secretion. Curr Biol (2010) vol. 20 (20) pp. 1840-5
  • Swoger et al. 4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies. J. Biophoton. (2010) pp. n/a-n/a
  • Keller et al. Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy. Nat Meth (2010) vol. 7 (8) pp. 637-42
  • Wohland et al. Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments. Opt Express (2010) vol. 18 (10) pp. 10627-41
  • Herbert et al. Arterial-Venous Segregation by Selective Cell Sprouting: An Alternative Mode of Blood Vessel Formation. Science (2009) vol. 326 (5950) pp. 294-298
  • Martinez-Morales et al. ojoplano-mediated basal constriction is essential for optic cup morphogenesis. Development (2009) vol. 136 (13) pp. 2165-2175
  • Keller et al. The zebrafish digital embryo: in toto reconstruction of zebrafish early embryonic development with digital scanned laser light sheet fluorescence microscopy.  (2009) pp. 73670G-73670G-10
  • Keller et al. Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy. Science (2008) vol. 322 (5904) pp. 1065-1069
  • Scherz et al. High-speed imaging of developing heart valves reveals interplay of morphogenesis and function. Development (2008) vol. 135 (6) pp. 1179-87
  • Huisken and Stainier. Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM). Opt Lett (2007) vol. 32 (17) pp. 2608-10
  • Arnaout et al. Zebrafish model for human long QT syndrome. P Natl Acad Sci Usa (2007) vol. 104 (27) pp. 11316-21
  • Engelbrecht et al. Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM). Opt Express (2007) vol. 15 (10) pp. 6420-6430

A detailed review on the use of SPIM in the field of developmental biology can be found here:

  • Huisken and Stainier. Selective plane illumination microscopy techniques in developmental biology. Development (2009) vol. 136 (12) pp. 1963-75

Currently a number of labs are using SPIM for their research on organogenesis in zebrafish inclunding: