Blog from January, 2021

A research assistant/lab manager position is immediately available in the Yin laboratory at Cincinnati Children’s Hospital Medical Center, Division of Gastroenterology, Hepatology and Nutrition. We use zebrafish to study liver development and pediatric liver diseases. Projects utilize multidisciplinary approaches: genetics (transgenesis and application of CRISPR/Cas9 genome editing), confocal imaging, chemical screen, bioinformatics, and liver histopathology.  


Details of the lab can be found at: 

http://www.cincinnatichildrens.org/research/divisions/g/gastroenterology/labs/yin/default/ 


We seek a highly motivated and organized research assistant/lab manager to conduct independent and directed scientific studies.  


Tasks include:  


Design and perform experiments according to established protocols, collect and analyze data, and trouble shoot.  

Develop, set up, and verify new procedures in the lab. 

Assist in training and mentoring postdoctoral fellows, graduate students, and junior laboratory personnel in laboratory techniques and equipment. Provide material, animal, and lab safety training according to regulations. 

Management of the zebrafish colony, maintain various lines.  

Manage laboratory supplies, reagents, constructs, and equipment.  

Effectively communicate with team members and contribute to their growth by sharing knowledge, experience, and skills. 


Requirement: 


Master degree in biology related field with or without experiences, or bachelor degree in biology related field with at least 2 years of experiences. 

At least a two-year commitment. 

Skills in molecular biology, immunohistochemistry, and confocal imaging are highly preferred. Experiences with zebrafish and mouse models are plus but not required. 

Strong time management and communication skills. Attention to detail. 


Interested applicants may send CV to: 


Chunyue Yin, PhD 

chunyue.yin@cchmc.org  

  

 Cincinnati Children's Hospital Medical Center is an Affirmative Action/Equal Opportunity Institution.  Women and Minorities are encouraged to apply. 

To apply: https://career4.successfactors.com/sfcareer/jobreqcareerpvt?jobId=44982&company=C0000274692P&st=2C808962685E767925326EFDE222835EA220E18A


We are looking for a highly motivated Research Assistant to join our group to investigate how the cardiac vasculature system develops and how it supports the regeneration of heart tissue after injury. The successful applicant will join Michael Harrison’s lab (labharrison.com) in Cardiovascular Research Institute (https://cvri.weill.cornell.edu/) at Weill Cornell Medicine in New York.


The lab is focused on both components of the cardiac vasculature, the coronary vessels that supply the heart with blood and the lymphatic vessels that remove fluid and debris from cardiac tissue.  We primarily study zebrafish which have enhanced cardiac repair compared to mice and humans. We want to identify dormant pathways and processes that can be utilized to accelerate regeneration in patients.


We utilize novel imaging techniques and zebrafish genetic models to dissect the cues driving vascularization and vascular mediation of heart regeneration. 


Successful candidates will primarily help with:

  • Management of the zebrafish colony including breeding, screening transgenic embryos, tracking fish numbers and tank densities. Work with the institute’s animal husbandry team that provide feeding and maintain the aquarium system.


  • Genotyping (PCR and electrophoresis)


  • Basic bench techniques including preparing solutions, sterile technique.


  • Histology – sectioning and staining tissues


  • Making transgenic and mutant zebrafish – including molecular biology and genome editing techniques, embryo microinjection.


  • Imaging – whole mount, live, confocal and tissue sections


  • Sequencing – preparing and analysis of bulk and single-cell sequencing samples


  • Assist in basic laboratory tasks and ordering supplies


  • Ensuring that safe laboratory practices are followed, including the use and disposal of chemicals and hazardous materials.


Experience with molecular biology, imaging, or zebrafish handling highly desired.


Education

Bachelor's or Master's Degree in biology or related field.

Minimum of 1 year of wet lab bench (academic or industry) experience preferred.

At least a two-year commitment preferred due to a substantial training effort associated with the position.


Knowledge, Skills and Abilities

Demonstrated organizational skills and ability to pay close attention to detail.

Motivation to learn and grow in scientific expertise.

Ability to manage work with minimal supervision.

Ability to trouble-shoot technical issues.

Strong communication and presentation skills.

Ability to work on multiple projects simultaneously.

Ability to plan and prioritize work while responding flexibly to rapidly changing priorities.

Ability to work flexible hours.

This position may require standing or sitting for long periods of time, including in a warm aquarium environment.



A fully funded postdoctoral position is available to study epigenetics of development using zebrafish as well as cavefish, mouse, and cell culture models in the laboratory of Dr. Brant Weinstein in the NICHD Division of Developmental Biology (DDB) in Bethesda, Maryland.  The Weinstein laboratory uses a variety of cutting-edge molecular, cellular, genetic, transgenic, microscopic imaging, and next-gen sequencing approaches to study the role of epigenetics during development.  In addition to other related studies, the laboratory is currently carrying out the first-ever genetic screen for vertebrate tissue specific epigenetic regulatory genes using a novel “EpiTag” transgenic epigenetic reporter line.  The scientific environment, resources, and stipend support for this position are superb.  Learn more about research in the Weinstein lab.


Interested applicants should have a Ph.D. or M.D. and less than 3 years' postdoctoral experience. To apply, send a curriculum vitae, bibliography, cover letter with a brief description of research experience and interests, and the names of 3 references (with phone numbers) via e-mail to weinsteb@mail.nih.gov and to amy.parkhurst@nih.gov.


The NIH is dedicated to building a diverse community in its training and employment programs.

A fully funded postdoctoral position is available to study the developmental and function of the brain meninges and/or the neurovascular unit in the zebrafish in the laboratory of Dr. Brant Weinstein in the NICHD Division of Developmental Biology (DDB) in Bethesda, Maryland.  The Weinstein laboratory uses a variety of cutting-edge molecular, cellular, genetic, transgenic, microscopic imaging, and next-gen sequencing approaches to study the meninges and meninges-associated cell types and the brain neurovascular interface.  The scientific environment, resources, and stipend support for this position are superb. Learn more about research in the Weinstein lab.


Interested applicants should have a Ph.D. or M.D. and less than 3 years' postdoctoral experience. To apply, send a curriculum vitae, bibliography, cover letter with a brief description of research experience and interests, and the names of 3 references (with phone numbers) via e-mail to weinsteb@mail.nih.gov and to amy.parkhurst@nih.gov.


The NIH is dedicated to building a diverse community in its training and employment programs.

A fully funded postdoctoral position is available to study vascular development, angiogenesis, and lymphangiogenesis in the zebrafish in the laboratory of Dr. Brant Weinstein in the NICHD Division of Developmental Biology (DDB) in Bethesda, Maryland.  The Weinstein laboratory uses a variety of molecular, cellular, genetic, transgenic, microscopic imaging, and next-gen sequencing approaches to study the specification, patterning, and differentiation of developing blood and lymphatic vessels.  Some of our current areas of research interest include endothelial specification and differentiation, assembly of vascular networks and the cues that guide their patterning; morphogenesis of vascular tubes, regulation of vascular integrity, and isolation and molecular characterization of novel vascular-specific mutants. The scientific environment, resources, and stipend support for this position are superb. Learn more about our current research.


Interested applicants should have a Ph.D. or M.D. and less than 3 years' postdoctoral experience. To apply, send a curriculum vitae, bibliography, cover letter with a brief description of research experience and interests, and the names of 3 references (with phone numbers) via e-mail to weinsteb@mail.nih.gov and to amy.parkhurst@nih.gov.


The NIH is dedicated to building a diverse community in its training and employment programs.

The HOUART lab, situated at the heart of LONDON, is seeking to appoint three postdoctoral research associates (PDRA) to join a WELLCOME TRUST-funded research programme aiming to understand the role of axonal splicing factors and intron retaining transcripts in neuronal maturation and degeneration. Our nervous system crucially relies on local fast decisions taken at synapses, long distance from the neuronal cell body. The mechanisms by which these decisions are spatio-temporally controlled remain obscure. Axonal splicing factors and intron-retaining mRNAs have newly been identified as key players in the process. We seek to understand the fundamental molecular and cellular roles they play locally in the dynamic control of neuronal connectivity, using animal models (zebrafish) and cell culture approaches.  

The research programme will require expertise in zebrafish genetics, transcriptomics, proteomics, high-resolution (live and fixed) molecular imaging, and sophisticated computational analytical methods. We wish to appoint one PDRA with expertise in cutting-edge high-resolution/molecular imaging technologies, a PDRA with strong zebrafish neurobiology background and a PDRA with very strong protein/RNA biology experience.  

Prof. Corinne Houart is a member and Deputy Head of the outstanding Centre for Developmental Neurobiology and MRC Centre for NeuroDevelopmental Disorders at King’s College London and attached member of the Francis Crick Institute. This rich research environment provides to the posts world-class research facilities.  

Deadline for application 28th February 2021. For further information, please contact Prof. Corinne Houart by email: corinne.houart@kcl.ac.uk

The Zebrafish Aquaculture Facility at New York University School of Medicine has a position available for a Research Associate starting in 2021.

The selected Research Associate will work in a team setting to support scientific research in the labs of Drs. Holger Knaut (https://www.knautlab.org/) and Jesús Torres-Vázquez (https://med.nyu.edu/faculty/jesus-torres-vazquez) at the Skirball Institute of Biomolecular Medicine, New York University.

Responsibilities include oversight of our 1000 square feet 3500-tank zebrafish aquaculture facility, breeding, and rearing zebrafish, maintenance/repair of the water system and assistance with experiments investigating the genetic regulation of zebrafish development, particularly the formation of the vascular system. The position is ideal for those aiming to get laboratory experience prior to pursuing a higher degree.


ELIGIBILITY (apply if you meet all of these criteria):

  1. B.S. or M.S. in Biology, Fisheries or related fields.
  2. Availability to start this position between April and June 2021.
  3. Commitment to the job for at least two years.
  4. Highly desired attributes include:
  5. a) Aquaculture experience.
  6. b) Practical knowledge of plumbing and electrical repairs.
  7. c) Research experience (laboratory or field).
  8. d) Excellent time management ability.
  9. e) Capacity to work alone and with others.
  10. f) Strong verbal and written communication skills.

The job involves frequent communication with members of both zebrafish laboratories and the NYU Facilities/Maintenance staff.


NYU School of Medicine offers an excellent salary (starting at $42,000) and benefits package.

 

TO APPLY

  1. Please send an e-mail to holger.knaut@med.nyu.edu using the subject line: "2021 Zebrafish Research Associate: your name".
  2. Attach your CV (entitled "2020 Zebrafish Research Associate: your name") as a single WORD or PDF file. Please start your document with the following information at the top. Without this information the review of your application will be delayed:
  3. Name, current living address  (city, state, and country) and telephone number.
  4. Expected date of availability.
  5. Degree(s), date(s) awarded and GPA(s).
  6. Name, title, e-mail and phone number of three references (please state in what capacity these references know you and for how long).
  7. Summary of experience: A paragraph (10 lines maximum) describing your interests and experience relevant to this position.



A postdoctoral position is available in the Cell and Developmental Biology Center (Rosa Puertollano, PI) at the National Heart, Lung and Blood Institute, National Institutes of Health. The successful candidate would join a multi-disciplinary and highly interactive team of researchers with diverse backgrounds and expertise in a collaborative scientific environment. Our laboratory is interested in understanding the contribution of lysosomes to different physiological processes, both in normal and pathological conditions. We use a wide range of cell biology techniques (molecular biology, protein biochemistry, confocal microscopy) as well as in vivo animal models (mouse and zebrafish). The laboratory has recently generated several zebrafish mutant lines carrying mutations in genes associated with lysosomal diseases and regulation. The Postdoctoral Fellow will be responsible for planning, initiating and conducting experiments with zebrafish to further characterize these lines. Prior experience in zebrafish biology, gene editing, and familiarity with high-throughput chemical screening assays is preferred. For more information, please visit: http://www.nhlbi.nih.gov/research/intramural/researchers/pi/puertollano-rosa/puertollano-rosa.html


Applicants should send their curriculum vitae, a brief statement of research interests, and the names and contact information of three references to:


Rosa Puertollano Ph.D.

Senior Investigator–Laboratory of Cell Biology

Building 50, Room 3537

50 South Drive, Bethesda, MD 20892-1454, USA

Email: puertolr@mail.nih.gov

Job title: Research specialist

Institution: University of Wisconsin - Madison

City: Madison

State: Wisconsin

Primary category: Research

Type of position: Research specialist


Job description

The Kang Lab (https://kang.crb.wisc.edu/) in the Department of Cell and Regenerative Biology is seeking a motivated and enthusiastic Research Specialist to perform biological research at UW-Madison. The primary duty for this position is to manage our zebrafish facility which includes daily feeding, water quality monitoring, zebrafish strain maintenance and shipping. The candidate will also participate in various research activities, including molecular, cellular and microscopy techniques, to investigate tissue regeneration using zebrafish as a model. To learn more about this position and to apply please visit: https://jobs.hr.wisc.edu/en-us/job/508211/research-specialist


Minimum Required Education: Bachelor’s degree

Minimum Required Experience: 1yr lab experience

Preferred Education: Biology or related field


Contact person: Junsu Kang, junsu.kang@wisc.edu

Link: https://jobs.hr.wisc.edu/en-us/job/508211/research-specialist

To apply: https://careers.peopleclick.com/careerscp/client_medcollegewi/external_staff/gateway.do?functionName=viewFromLink&jobPostId=17827&localeCode=en-us

Purpose

Dr. Lam’s laboratory (https://www.mcw.edu/departments/cell-biology-neurobiology-and-anatomy/people/pui-ying-penny-lam-phd) is broadly interested in small molecule tool development along with the use of these tools and in vivo imaging to interrogate biological processes in health and disease.  Specific research directions include developing novel chemo-optogenetic actuators for in vivo applications and studying innate immune cell biology.  Zebrafish and Danionella translucida are used as animal models.  The lab is looking for a motivated Research Technologist to provide support for lab research through managing the lab, preparing reagents/media, maintenance and communication of data, and execution of laboratory experiments.  While experience in molecular and cellular biology, microscopy and working with zebrafish is welcome, hands-on training will be available for all required tasks.  The ability to work independently is highly desirable.  Salary is commensurate with experience.

Primary Functions

  •  Carry out experimental designs for sponsored research projects, and perform routine experiments including but not limited to plasmid preparation, cloning, microinjection, genotyping, in vivo imaging using fluorescence microscopes and small molecule screening.

  •  Record and compile accurate records and communicate summaries of experimental results.

  •  Assist with the evaluation, interpretation and assessment of experimental data and quality control of assays.

  •  Participate in meetings with principal investigator and research staff and evaluate/interpret the validity of data as needed.

  •  Will have opportunities to be included in publications.

  •  Carry out assigned duties for ordering and maintaining supply of inventories.

  •  Operate, calibrate and perform routine maintenance on specialized equipment used in research.

  •  Prepare media, buffers, reagents, culture plates and competent cells etc.

  •  Manage and maintain zebrafish and Danionella translucida lines.

  •  Oversee daily lab operation in the absence of the supervisor or Principle Investigator.

 

Knowledge – Skills – Abilities

Knowledge of biology, chemistry, mathematics, documentation, and records management.

Data utilization, complex problem solving, critical thinking, resource management, and writing skills.

Preferred Schedule:
8am-5pm with flexibility as required for experiments

Position Requirements:

Specifications

Appropriate education and/or experience may be substituted on equivalent basis

Minimum Required Education:               Bachelor’s degree

Minimum Required Experience:             Undergraduate lab experience

Preferred Education:  Master in biology or related field

Preferred Experience:  Zebrafish or confocal imaging experience is a plus

Field:  Cell and molecular biology, immunology, neuroscience

About MCW
The Medical College of Wisconsin (MCW) is one of the largest healthcare employers in Wisconsin. We are a distinguished leader and innovator in the education and development of the next generation of physicians, scientists, pharmacists and health professionals; we discover and translate new knowledge in the biomedical and health sciences; we provide cutting-edge, collaborative patient care of the highest quality; and we improve the health of the communities we serve.

We are an Equal Opportunity Employer and do not discriminate against any employee or applicant for employment because of race, color, sex, age, national origin, religion, sexual orientation, gender identity, status as a veteran, and basis of disability or any other federal, state or local protected class.

https://jobrxiv.org/job/emory-university-school-of-medicine-27778-research-technician-lab-manager-zebrafish-skeletal-biology/

Emory University School of Medicine

The Henke lab at Emory University is looking to hire a motivated research technician to join our group to investigate gene function during development, homeostasis and disease of the musculoskeletal system using the zebrafish as a model. We utilize the CRISPR/Cas9 system to generate new genetic tools; phenotyping by microCT, histology and enzymatic stains; as well as transcriptomic profiling. Responsibilities will include collecting, organizing and reporting research data under minimal supervision; assisting in the design of experiments; maintaining laboratory supplies and equipment; instructing others in basic laboratory techniques; collecting and summarizing data; monitoring results; reporting unusual outcomes. Necessary skills include organization, critical thinking, communication skills, standard lab notebook practices; attention to detail.

Required education and experience: BS or BA in biological sciences required, experience with basic programming and working with zebrafish are a plus.

To initially apply, please send your CV and a brief description of why your are interested in joining the lab. We will send out an official application link if we feel you are a good fit.

Tagged as: Life Sciences

The lab of Professor Geoff Goodhill (http://www.goodhill.org/ ) is relocating from Australia to the departments of Developmental Biology and Neuroscience at Washington University in St Louis. We are looking for a Research Assistant to help support the lab's research program examining behavior and neural circuits in larval zebrafish, including autism mutants. These experiments include high-speed imaging of hunting behavior, and 2-photon and light sheet microscopy to record neural activity via fluorescent calcium indicators.

To apply please go to jobs.wustl.edu and search for job number 50055. Candidates with at least a bachelor's degree in a scientific discipline and prior experience in relevant laboratory techniques are encouraged to apply. Preferred qualifications are:
Experience in animal care and handling, ideally including zebrafish breeding, feeding and washing of embryos, maintaining live fish food, and genotyping.
Experience with fluorescence microscopy.
Experience with managing lab supplies and expenses.
High level of organization, communication and interpersonal skills.
Adaptability and ability to manage multiple tasks.
Working knowledge of standard computer software and general lab equipment.

Pay will be commensurate with experience.

The lab of Professor Geoff Goodhill (http://www.goodhill.org/ ) is relocating from Australia to the departments of Developmental Biology and Neuroscience at Washington University in St Louis. We are looking for postdocs to help drive the lab's research program examining behavior and neural circuits in larval zebrafish, including autism mutants. These experiments currently include high-speed imaging of hunting behavior, and 2-photon and light sheet microscopy to record neural activity via fluorescent calcium indicators. The lab emphasises a tight integration of theory, experiment and sophisticated data analysis, and you will be working closely with people from backgrounds such as math, physics and engineering.

Washington University in St Louis is ranked in the top 10 globally for Neuroscience and Behavior, and offers an outstanding intellectual environment for neuroscience research.

To apply please contact Prof Goodhill, including a detailed CV and cover letter explaining your interest: g.goodhill@uq.edu.au

The Zebrafish Facility and Solnica-Krezel lab is seeking applicants for a Research Technician II in the Department of Developmental Biology at Washington University School of Medicine.

Washington University in St. Louis is home to one of the largest zebrafish facilities in the world and with robotic feeding and cleaning systems, it is the world’s most modern. The facility allows large-scale, collaborative projects that would not be possible for individual investigators, helping scientists understand human development and disease, from birth defects and cancer to muscle and nerve disorders.

As part of the research services aspect of the Zebrafish Facility, we are looking for a research technician with experience in molecular biology, that will assist with the carrying out of specific experiments that are available as a service to zebrafish investigators (mutant lines generation using genome editing approaches, microinjections, and genotyping) as well as general husbandry duties.


To learn more about this position and to apply please visit: Research Technician II - Developmental Biology - 50051


Or visit: jobs.wustl.edu and search 50051 in Keywords