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Make 500xE2B by dissolving 11.0 g CaCl2 (or 14.6g CaCl2 x 2H2O) in the final volume of 200 ml
- add Millipore water to 200 ml
- shake to dissolve the reagent
- autoclave
- aliquot into 20 ml portions (in 50 ml Falcon tubes)
- store at -200C
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