E2B

Owned by nathan

Last updated: Jan 18, 2018 by Anne Eagle

1 min read

Make 500xE2B by dissolving 11.0 g CaCl2 (or 14.6g CaCl2 x 2H2O) in the final volume of 200 ml

add Millipore water to 200 ml
shake to dissolve the reagent
autoclave
aliquot into 20 ml portions (in 50 ml Falcon tubes)
store at -200C