E2B

Owned by nathan
Last updated: Jan 18, 2018 by Anne Eagle

Make 500xE2B by dissolving 11.0 g CaCl2 (or 14.6g CaCl2 x 2H2O) in the final volume of 200 ml

  • add Millipore water to 200 ml
  • shake to dissolve the reagent
  • autoclave
  • aliquot into 20 ml portions (in 50 ml Falcon tubes)
  • store at -200C

 

 

 

 

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