FIXATIVES

Owned by nathan
Last updated: Jan 18, 2018 by Anne Eagle
1 min read

Fix buffer:
4% sucrose
0.15 mM CaCl2
0.1 M PO4 pH 7.3.

For general fixation:
1.5% glutaraldehyde, 0.5% paraformaldehyde in fix buffer.

For antibody labeling:
4% paraformaldehyde in fix buffer.

*FREEZING MEDIUM

WITHOUT Methanol*

Ginsburg Fish Ringers 10 ml (room temp.)
Powdered Skim Milk 1.5 gm

WITH Methanol

Ginsburg Fish Ringers 9 ml (room temp.)
Methanol 1 ml
Powdered Skim Milk 1.5 g

 

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