PTU-2

Owned by f.j.vaneeden@sheffield.ac.uk
Last updated: Nov 07, 2011Version comment

Freeks recipe, no heating or bulky storage requirement:

Stock 7.5% PTU in 100% DMSO (0.75g per 10ml), store at 4C

use 40 ul per 100ml embryo medium to get 0.003%


I use +/- 9ul per petridish, swirl immediately after adding.

Wear gloves!!, DMSO helps PTU penetrate your skin