E2C
Make 500xE2C by dissolving 6.0g NaHCO3 in the final volume of 200 ml
- add Millipore water to 200 ml
- shake to dissolve the reagent
- autoclave
- aliquot into 20 ml portions (in 50 ml Falcon tubes)
- store at -200C
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Make 500xE2C by dissolving 6.0g NaHCO3 in the final volume of 200 ml
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