Chemical Chemical Concentration
Name Formula (mM)

Hepes C8H18N2O4S 10.0
Potassium Chloride KCl 80.0
Sodium Chloride NaCl 45.0
Sodium Acetate C2H3NaO2 45.0
Calcium Chloride CaCl2 0.4
Magnesium Chloride MgCl2 0.2

Bring up to volume with ddH2O, and adjust to pH 7.7 with 1M NaOH. Filter through 0.22μm filter, and store at 4ºC.