FIX BUFFER

Dilute 2x fix buffer 1:1 with dH2O.

Check pH. Adjust if necessary to 7.3 with 1 M NaOH or HCl. Add dH2O to a final volume of 15 ml.

1.5X Fix Buffer

1.0 g Sucrose
18.75 μl 0.2 M CaCl2
5 ml 0.5 M PO4 buffer, pH 7.3

2X Fix Buffer

8.0 g Sucrose
0.15 ml 0.2 M CaCl2
90 ml 0.2 M PO4 buffer, pH 7.3

Check pH. Adjust if necessary to 7.3 with 1 M NaOH or HCl. Add 0.2 M PO4 buffer to a final volume of 100 ml