E2A Stock

Owned by nathan
Last updated: Jan 18, 2018 by Anne Eagle
1 min read

Prepare 100xE2A by dissolving the following ingredients in a final volume of 1600 ml:
140.0g NaCl
6.0g KCl
19.2g MgSO4
3.3g KH2PO4
1.1g Na2HPO4

  • add Millipore water to 1600 ml
  • shake and stir to dissolve the reagents
  • autoclave
  • stir O/N to dissolve any precipitation that has formed during autoclaving
  • store at 40C

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