WHOLE-MOUNT IN SITU HYBRIDIZATION SOLUTIONS
PBST PBS plus 0.1% Tween
SSCT SSC plus 0.1% Tween
HYB* 50% formamide
5xSSC
0.1% Tween-20
HYB+ HYB* with:
5 mg/ml torula (yeast) RNA
50 μg/ml heparin
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PBST PBS plus 0.1% Tween
SSCT SSC plus 0.1% Tween
HYB* 50% formamide
5xSSC
0.1% Tween-20
HYB+ HYB* with:
5 mg/ml torula (yeast) RNA
50 μg/ml heparin
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