WHOLE-MOUNT IN SITU HYBRIDIZATION SOLUTIONS

PBST PBS plus 0.1% Tween

SSCT SSC plus 0.1% Tween

HYB* 50% formamide
5xSSC
0.1% Tween-20

HYB+ HYB* with:
5 mg/ml torula (yeast) RNA
50 μg/ml heparin