(Source: C. Walker from Zebrafish Book 5th Edition)
Zebrafish eggs are fertilized outside the body making it possible to manipulate the process to advantage. For example, haploid embryos can be produced by fertilizing eggs with sperm that have been irradiated with ultra violet light (UV sperm) destroying the sperm DNA so that it cannot contribute genetically to the embryo but leaving the sperm intact and able to activate the eggs. Haploid embryos develop from the female genetic complement only. Any mutation carried by the female will be seen in her haploid offspring. Additionally, it is possible to make these haploid embryos diploid by application of pressure or heat shock during early developmental stages. This section provides an overview of these genetic methods. Detailed procedures follow in a later section.
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