(Source: R. Andrews and T. Ellis from Zebrafish Book 5th Edition)
This method allows the production of homozygous haploid embryos for screening for recessive mutations. Haploid embryos develop fairly normally for the first few days, but eventually develop bent tails and vacuolated body cavities. They are inviable.
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This is a general procedure for making infertile sperm that can still activate eggs for production of haploid, early pressure (EP) or heat shock (HS) embryos.
Materials Needed:
Watch glass
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