(Source: G.E. Corley‑Smith, C.J. Lim, and B.P. Brandhorst from Zebrafish Book 5th Edition)
(In The Zebrafish: Genetics and Genomics, H.W. Detrich, M. Westerfield, and L.I. Zon, eds., San Diego: Academic Press, Meth. Cell Biol. 60:45‑60, 1999)
Overview of procedure
...
Confirmation of exclusive paternal inheritance requires investigating the inheritance of parentally polymorphic DNA markers to putative androgenetic progeny. The lack of homozygous maternally specific markers in the progeny is strong evidence supporting sole paternal inheritance, although it does not rule out the possibility of some maternal leakage.
Reference
Hertwig, O. (1911) Die Radiumkrankheit tierischer Keimzellen. Arch. Mikr. Anat. 77:1‑97.
Acknowledgment
We are indebted to Charline Walker for the *AB line of fish, and for her extremely helpful advice on in vitro fertilization and on heat shocking zebrafish eggs.