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Zebrafish Genetic Map: Primers Page

This table contains the primer sequences for the microsatellite markers on the zebrafish genetic linkage map, as published in Nature Genetics: A Microsatellite Genetic Linkage Map for Zebrafish (Danio rerio), Ela W. Knapik, et al.
The primers are available from Research Genetics.

Primer

LG

Forward sequence

Bases

Reverse sequence

Bases

PCR Product Size

BMP4

17

CTGTGAGGAGCTTCCATCACGAA

23

GGAATCGGCAGAGGCTGTCTCC

22

160

msxD

21

AGCTCCGTCTTCACTGACTGACA

23

TGTGGTGGACATTAACTCATCCA

23

240

Z4

14

TCAGAGGAGCGCATGAAAGT

20

CTGAAAACACCCATCACGTG

20

119

Z7

19

TCATGAGCAGCGTTTTTCC

19

AAAGCACCATCCCACAAAAC

20

145

Z13

5

CACGTGCTGATGCAGGAC

18

GATGACAGCCAATCAGAGCA

20

144

Z16

21

ATACTCAAACACATCCCAGCC

21

GAGGTGCACGTCAGCGAC

18

150

Z18

21

CTCTTTTGCCTCCTCTCGG

19

CAGCCTTAACACTGTGTCTGTG

22

147

Z23

2

TGTAGATTAATGCTTTTCTGTGCA

24

GCATATGAAGCAGGTCTGCA

20

133

Z24

15

CACCTTCACGGTGAGTAGCA

20

GTGGAATGGTGTGACTAATGTCA

23

149

Z28

13

TCTGGTGGACTCGCTGAAG

19

AAGTAAGCGCCACCAGAGTG

20

141

Z37

15

GGTGTTCGCGACCAAGTC

18

TTTGCGTGGAATTTGAACTG

20

83

Z46

2

GCTGTGTGTGCCTCATCTGT

20

GCAGACGCTGTACTCCATCA

20

224

Z49

25

GATTATCCTCATAAGTACGGGCC

23

AGCACATCTTCATCCTGCG

19

216

Z106

9

GAGACCGACCGTCTGCTAAC

20

TCCAGAGAACACCCTGCTG

19

187

Z130

23

ACACGCTGGAGAGCCAAC

18

CCAGAACCCACCTGGGAC

18

104

Z140

8

ATTTGGCAGATTCCTTCATCA

21

GGAACACCTGTGCCCGAG

18

213

Z160

19

CGCTACCTGAAGGACACCAT

20

CTGCTATTGCTATACTTGTGGGG

23

240

Z164

1

TTGCTGTGTGTGTTTCTCAGTG

22

TGTTCCATCGCTGAGAAGTG

20

221

Z170

8

CTGAAAGTCTTCACATCTGTGACA

24

GAATAAAGGTGGGGGGTGTT

20

121

Z176

23

CTCCAGAACAGGTTAGACACACC

23

CTGAACACCTCGAACCTTTACC

22

219

Z177

7

GGAAACTCTCACGCTCGTTC

20

ACGGATGTTCCTGTAAACGC

20

143

Z178

22

ACCACAAGCGTGTGAGTCTG

20

TTGACTGTGGCCTGCTGTAG

20

108

Z191

7

AATATAGCGAAGGGACGATCC

21

GAGGACTTCAGCAGAAACCG

20

123

Z230

22

GGTTCTCCCACCAGCACTAA

20

CGACTCAGACTTACTGGTGTGC

22

141

Z233

25

CAGTTTTCGGGACCTCTGAG

20

TGGATGAAGTTTACGGAGCC

20

148

Z241

24

CAATTAAAGGTGCAGTGGAGC

21

TTGGCCTCTGCTGTTTCTCT

20

231

Z247

12

GATTTACCTGTTTCACAGCATCC

23

CATCCTGAGATGTCATCTGATAGA

24

242

Z249

24

TCTTCCCCTACAGGCACAGT

20

ATGATACGCAGTCAACGTATCG

22

147

Z265

6

CCTTCCCTCAAAGAGCGAG

19

TGGAGTCGCTCATTAGCAAA

20

113

Z266

17

TCCGGCTGACATGAGGAAT

19

ACGCTCTGCTTCATGGTCTT

20

212

Z342

23

CATTGAGTTTAGACCCCCACA

21

TCACTTCTGCTTTATAAGGCACC

23

163

Z355

10

CAGCCTGAGCACACAGAGAC

20

GCACAGGCCTAATAAGACGC

30

204

Z374

20

TTTTAATCCAGCAGCAGATCTG

22

CCCAGGTTCACAAACCCTC

19

166

Z379

5

AGCCTTTCACTGGCCCTATT

20

CAAGGTGGAGTAAAAATCCTGC

22

222

Z381

17

AAACGTTGTGATGTGATATTCCC

23

TCTCCAGAAGCCTCGTGTG

19

205

Z382A B

7 7

GTGTCCGTTTTTTCGGTGTT

20

TAGCACGCTAACGGCTAACA

20

104

Z419

3

ATTATCCAGCACATGACGAGG

21

TCTCGCTCCTCACCATCAC

19

120

Z450

1

AAGTACAGCATTGTCCACAGTCA

23

CAGGGAAACTGCTGATGTTG

20

154

Z464

25

ACCTCCACACGGAAAGGAG

19

GGTGATGAAAAATTAGCCTTCG

22

156

Z470

15

GAGAGCCGAGGTGTGTATGC

20

CACTGCCTATTACACCACTGATG

23

139

Z531

10

GCAGGTTGCTCTGGTTTCTC

20

TTTCCGATGACAGATCGTGA

20

118

Z536

20

ATCCAGAGTGTGTCCCAGTTG

21

AGGTGTGTCCCACTGACTCC

20

118

Z540

21

GTGGCCGTCAGTTCAGCT

18

CCTCCTGTCAGGTGAAGCTC

20

127

Z553

19

GGCGTAATCCTCATTCCTGA

20

CAAACTGTGTTGTGTGTTTTTCTG

24

209

Z559

9

ACCCGGCTGTAGTGAAACC

19

CCTCTCTGCCCTCCTCTTG

19

114

Z562

9

TGTACTTCTCTCTCAATACACCGG

24

AGGCCTCTGATTAACTGAATTACA

24

207

Z618

14

CCTTTCACACTATACAGCGTATCA

24

TCTTCTGGGGTTTACTGCTCA

21

165

Z643

23

AGGCTGGGCTATAATGCTGA

20

TAACGAGTCTGTGTTTCGCG

20

241

Z644

2

CACGGACAATCCAGTGTACG

20

GCAACAGTATAAGACTGTCTTGGG

24

144

Z647

5

GTGAGAGGTACGGCAGGAGA

20

AAACAACCCTGCATCCACTC

20

249

Z652

17

AGAGAGTGAGCTCTGGAACCC

21

CACTCTTGAAGATGGCAGAGG

21

132

Z669

22

GTGTGTGATGTTGTGTTTGTCG

22

GGGGTCACTTCTGTTTACAAGC

22

137

Z684

22

GCTACAGCTCGCCTGTGTG

19

ATCCGATACAGCCCGAGTC

19

89

Z685

11

GGTGCTCGCAGGTGTTAATT

20

CCAGGGCGTGATGTACTTTT

20

204

Z697

14

AAAGTGTTACCGGGCAACAG

20

GGGTTAGCATGTGGGCTG

18

114

Z699

16

CTTGTGAACGTAGCCGTTGA

20

TAAAACCACACACGAGCAGC

20

209

Z720

23

ACTCTCAACATGAAGTGCTTTCC

23

ACAGGGAATGAGCTGAGAACA

21

145

Z722

21

AACTGAATGACTATGGGCGC

20

TCTTCTTGGTAACCAGACAAAGC

23

101

Z728

1

GTGGTCATGTGACACAGGATG

21

GCTCTTACAGTCCTGAGAACAGC

23

204

Z732

15

GTGCCTTCCGTTACCTTCAC

20

TTTTAAGGAAAAAAAGTGAATGCA

24

133

Z737

18

AACACTTGATATTGCATGACGG

22

ATGTTGGTTAGCATGCACCA

20

126

Z740

6

AAACTTTGCTGCTCTTCCTCC

21

CTCTGTCAGGTCCTGTGATCC

21

203

Z744

10

TATACTGCTTATTGGTGAAATCCG

24

CTGTACAGCCCAGACAAGAGC

21

149

Z787

25

GCTGCGTGATATTCATGGG

19

CTGTATCCTGCTATACAGCCCC

22

111

Z789

8

CTCCTGCTGGCCCAGTAAT

19

ACTGAACATTCAGGCTTAAATGC

23

203

Z793

6

TGGTGAAATTTTGGACAGCA

20

CAAGGACCAAATGGAAGGAA

20

205

Z811

16

CCATGTGGGTGGAAAAGACT

20

CTGTCTTTCAGTTTTGCTTATGTG

24

175

Z818

13

CATGGCTTTACTGCTCTGAGC

21

CTTGTGCTCAAACTGAACACTG

22

147

Z846

15

CCTCTCAGTCTCCATCACCTG

21

GTGTGTGCTATAATGCCATTGG

22

94

Z851

2

ACAGTCTTTACTCCGCAATAATCC

24

ACTGAAGAGGAGGACCAGAGC

22

142

Z852

17

CCAGACTAACCAGACCTGAACC

22

GAACATACCTGAACAGAAAACACG

24

215

Z854

9

TCTTCTGAATCCTCACTCAGAGC

23

CCATAATGCATTTGGGGAAG

20

139

Z858

15

CTTCCACATGGTCATTATGGG

21

AACCAGTGGAGCGACCAG

18

209

Z868

11

GATCTCCCTAATTATAGCACTCGC

24

TCATCGCATCTGCAGTCAG

19

128

Z870

8

TTCCGGCTCTCAGTGTCTTT

20

CCTGACCAGCATAGAGTCATG

21

193

Z872

3

CTTAAAGCCCAGTGAGGCAC

20

ATGCCTGGTAATGAGCAGCT

20

220

Z879

14

TGAGGAGGTTTGTCACCACA

20

TGAAAAAAACACCAAGCGC

19

203

Z880

6

TGATCTGAGACGTCTGCCG

19

GCGCTCTGACAGTGCTTACA

20

150

Z896

5

ACAACCACAGCATGACCTCA

20

ACAAGTGCTGCAGAAGATGTATG

23

139

Z911

24

GCTGCTTGTGGTGTGAAAAA

20

TAATTTCATTCCACCTGCTGG

21

126

Z916

9

GGATCTGTTCAGCCACTGAA

20

TCACCTGATCACCATGCAGT

20

150

Z928

18

CCAGAGTACTCGATCTGTCTTGA

23

ACACCTTCGGCAGTTTTCAC

20

134

Z937

12

AGAGCTAGCGCTGAGCTAACA

21

TCTCTCTGTTCATCAGCAGCA

21

137

Z938

22

CAGACGGACAAGTGGAGGAT

20

CAAATGCTTCTCACTTCCTCG

21

206

Z940

15

CTGCTAACCAAATGCAGAAGC

21

TAACCTGATGGAAAATCACACG

22

121

Z953

21

AGAAAAGAGTGAGGACTGTGTGC

23

TGCGAGTTGACAGCCTGTAC

20

98

Z963

3

ACTGGCACTTCACGCTCC

18

TCTCACACTGAGTGCTAAACGG

22

201

Z974

14

AGCTCCAGCCCTACAACTACC

21

TGAAGTTCAGACTAATGATCTCGC

24

206

Z975

25

ACCTGTGTCATACCCATGTCA

21

CTGGTGTCCCCCACAGTC

18

150

Z984

4

TTTCTCAGAGCACAACCTTTAAC

23

GAGAGCAAACTCCAGCTGCT

20

200

Z987

8

TCTGGTTTCACATTAACATGTGC

23

ATCTGCTATCGGCTCTGTTAGC

22

235

Z992

16

TTGACGTAGTTTGTGCGTTTG

21

CAATCAGAGCAGGAGGCG

18

150

Z1050

6

ACTCTGAGTCTGCAGTCCCG

20

AACTGCTGCAGTTAATTATTGC

22

145

Z1052

8

CCCATTGGAACTGAGACACCTT

22

ACAGGGGGGGCTAATAATTCTG

22

138

Z1059

7

AACAGGTGACAGAGCACACG

20

GGGAGAGGGCAGGACAATAT

20

150

Z1061

24

CATCGCTGGTTACTGCTGG

19

TCTCTATTATAAGCCTCCTCACCG

24

102

Z1068

8

GGTGAGAAACACAAGAGCAGC

21

CAGACCCAGATTCTTCAGCC

20

132

Z1136

18

TCATGTGAAGCCTGCAGTTC

20

AGCTTCAGCTGTTTAAAGGTGC

22

105

Z1138

6

GAACGACGGCACTCTATCCG

20

TTGCAGCCTTAGTGTAAACGAA

22

151

Z1140

3

CGCTTCAGCCTCAATCTCTC

20

TGTAGCTTTCTGGGCCTGAT

20

197

Z1141

12

TCTGTCCTGTGCGCAGAG

18

AGTTCCAGTGCAGACGGTG

19

138

Z1144

18

CTCCTCTCCGGGATACACAC

20

ACCACGAGTCTGAGTGCTGA

20

249

Z1145

10

GGGAACGACCCTTTTCAATT

20

TCCAAGTTGGCATAGATGTATCT

23

199

Z1146

12

ATGCCACCCCTCTCCTTC

18

TGCCAACATGCTAACAATATCC

22

144

Z1148

22

GGCTACACCTCAAACGCTTC

20

CAACACCTGATCAAACGCC

19

200

Z1154

1

TCATGATTGTTTGGAATGTAATAGTG

26

TTGAGCGGTAGTCTTCTACGC

21

130

Z1159

2

CGCTAATGACTAGCGTGACG

20

ATAACCACAGTGATAAACGCA

21

140

Z1162

21

TTTATTAATATTCTGTGCTGGGC

23

GCCGGAAAGACACCTTCATA

20

131

Z1167

5

TCCCTTTGTAACATCGGCTC

20

AAGCCCCGACTACTGTCTGA

20

189

Z1173

1

CAACAGTGATCACACCGACC

20

CTGCCCTTCTCTGAATCAGG

20

123

Z1176

12

GAGGATTTAATCATCATTTGCTCC

24

AAACACCACGATCAGCAGC

19

125

Z1179

12

CAGGGAACCGTCACATTCAGC

21

TCTGCAGACTCCCACTGACCC

21

198

Z1182

7

GAGCAGACACTTTACATTTTGTGG

24

GACACTTACAAAGAGTCTTTCATTTCC

27

147

Z1183

3

CCGCGGAAGATTAATCAGAC

20

AGATTCACTCCGTGCTCCAT

20

139

Z1185

3

CAGCAGGAACGAGGACGTCA

20

GGGTGGGGTTAGGGCAATAG

20

248

Z1191

3

TCAGAACTCCAGCCACCAG

19

TGGATATACTTTATCCCTAACTGAACG

27

146

Z1195

15

AACCCACACCGTCACATCGT

20

ATACGCCACTGACGAGAAGTGG

22

173

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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