Seok-Yong said...Hi,
I'm wondering if it's OK to use a T3 promoter on pDestTol2pA2 to generate an in situ probe upon completing 3-fragment gateway cloning (p5E-CMV/SP6 + pME-gene of interest + p3E-polyA).
T3 seems to be a bit apart from the stop codon of the gene of interest: two polyA's and Tol2 site in between.
I guess this extra sequence would not affect the in situ efficiency. Am I right?
Just curious! Thanks. SY
AUGUST 30, 2007 10:08 PM