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General Methods for Zebrafish Care
Breeding
Embryonic and Larval Culture
Imaging
Cellular Methods
Dissociated Cell Culture
Genetic Methods
•
Conventions For Naming Zebrafish Genes
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Suggested Guidelines For Maintaining Mutant Stocks
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Overview Of Methods For Parthenogenesis
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Production Of Haploid Embryos
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Production Of Homozygous Diploid Embryos
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Delayed In Vitro Fertilization Using Coho Salmon Ovarian Fluid
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Production Of Androgenetic Haploids And Diploids
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Freezing Sperm
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Thawing And Using Frozen Sperm For In-Vitro Fertilization
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A High-Throughput Method For Sperm Cryopreservation And In Vitro Fertilization
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Gamma Ray Mutagenesis
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Chemical Mutagenesis
•
ENU Mutagenesis Procedure With Increased Fish Survival
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Testosterone Treatment To Produce Males
•
Fin Amputations
•
Zinc Finger Nucleases - the Utah-Big Love Approach
Antisense Methods
Histological Methods
in situ Hybridization Techniques
Mapping
Transgenesis
•
Gene Cloning
DNA Analysis
RNA Analysis
Protein Analysis
•
Microarray
Recipes
Behavioral Methods
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Genetic Methods
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Zinc Finger Nucleases - the Utah-Big Love Approach
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Zinc Finger Nucleases - the Utah-Big Love Approach
grunwald@genetics.utah.edu
Jonathan Knight
Owned by
grunwald@genetics.utah.edu
Last updated:
Mar 04, 2011
by
Jonathan Knight
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ZFNPROTentirefeb2011.pdf
ZFNPROTentirefeb2011.docx
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