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Light sheet imaging (e.g. SPIM)

Light sheet imaging (e.g. SPIM)

May 11, 2011

Light sheet microscopy, most commonly refered to as Selective Plane Illumination Microscopy (SPIM), has been shown to perform extremely well in large, living embryos such a zebrafish embryos and larvae. Here is a list of references that demonstrate the use of SPIM in zebrafish:

  • Arrenberg et al. Optogenetic control of cardiac function. Science (2010) vol. 330 (6006) pp. 971-4
  • Liu et al. A dual role for ErbB2 signaling in cardiac trabeculation. Development (2010) vol. 137 (22) pp. 3867-75
  • Bagnat et al. Cse1l is a negative regulator of CFTR-dependent fluid secretion. Curr Biol (2010) vol. 20 (20) pp. 1840-5
  • Swoger et al. 4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies. J. Biophoton. (2010) pp. n/a-n/a
  • Keller et al. Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy. Nat Meth (2010) vol. 7 (8) pp. 637-42
  • Wohland et al. Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments. Opt Express (2010) vol. 18 (10) pp. 10627-41
  • Herbert et al. Arterial-Venous Segregation by Selective Cell Sprouting: An Alternative Mode of Blood Vessel Formation. Science (2009) vol. 326 (5950) pp. 294-298
  • Martinez-Morales et al. ojoplano-mediated basal constriction is essential for optic cup morphogenesis. Development (2009) vol. 136 (13) pp. 2165-2175
  • Keller et al. The zebrafish digital embryo: in toto reconstruction of zebrafish early embryonic development with digital scanned laser light sheet fluorescence microscopy.  (2009) pp. 73670G-73670G-10
  • Keller et al. Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy. Science (2008) vol. 322 (5904) pp. 1065-1069
  • Scherz et al. High-speed imaging of developing heart valves reveals interplay of morphogenesis and function. Development (2008) vol. 135 (6) pp. 1179-87
  • Huisken and Stainier. Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM). Opt Lett (2007) vol. 32 (17) pp. 2608-10
  • Arnaout et al. Zebrafish model for human long QT syndrome. P Natl Acad Sci Usa (2007) vol. 104 (27) pp. 11316-21
  • Engelbrecht et al. Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM). Opt Express (2007) vol. 15 (10) pp. 6420-6430

A detailed review on the use of SPIM in the field of developmental biology can be found here:

  • Huisken and Stainier. Selective plane illumination microscopy techniques in developmental biology. Development (2009) vol. 136 (12) pp. 1963-75

Currently a number of labs are using SPIM for their research on organogenesis in zebrafish inclunding: