Zebrafish Monoclonal Antibodies
- Jonathan Knight
- Anne Eagle
- ZFIN Admin
(Source: K. Larison and B. Trevarrow from Zebrafish Book 5th Edition)
Monoclonal antibodies that recognize a variety of structures in zebrafish embryos are available from the library at the Zebrafish International Resource Center. Antibodies can be ordered online at: http://zebrafish.org/. Please feel free to communicate with ZIRC if you have any questions: zirc@zebrafish.org
The following is a partial list of monoclonal antibodies raised against zebrafish immunogens at the University of Oregon. These are available from the Zebrafish International Resource Center (http://zebrafish.org/).
Antibody Name | Isotype | Cellular Binding Site | Anatomical Structure Labeled |
anti-DLX3b | IgG1,k | Â | border of neural plate, otic placode, otic vesicle, olfactory epithelium, median finfold, |
anti-VegT | IgG2a,k | Â | adaxial cells, lateral mesoderm, prechordal plate, tail bud |
zl-1 | IgG1,k | cytoplasm or extracellular matrix | lens fiber cells |
zm-1 | IgG1,k | granular cytoplasm | actinotrichia, pectoral fins, ventral fin fold, heart, musculature system, branchial muscles, enteric musculature, extraocular musculature, abdominal musculature |
zm-2 | IgG1,k | granular cytoplasm | actinotrichia, meninx primitiva, dorsal fin musculature |
zm-3 | IgG1,k | granular cytoplasm | musculature system, branchial muscles, enteric musculature, extraocular musculature, myotomes, somites, |
zm-4 | IgG1,k | Â | Â |
zn-1 | IgG1,k | cytoplasm | axons, blastomeres, dorsal root ganglia, enteric nervous system, sensory hair cells, neurons, primary motoneurons, peripheral nervous system, Rohon-Beard neurons, YSL |
zn-2 | Â | cell surface | ventral root, forerunner cell group, sensory hair cells, epibranchial placodes, lens, brain (neuropil), Rohon-Beard neurons, dorsal roots, trigeminal ganglion, trigeminal nerve (cranial nerve V), organs |
zn-3 | IgG2b,k | cell surface | cranial ganglia, meninx primitiva, enteric nervous system, pectoral fins, rhombomeres, retinal inner plexiform layer, neurons, brain (neuropil), retinal outer plexiform layer, peripheral nervous system, neurites |
zn-4 | IgG1,k | cytoplasm or cell surface | retinal neural layer, fins, retinal ganglion cell layer, sensory hair cells, rhombomeres, retinal inner plexiform layer, neural crest cells, neurons, brain (neuropil), epiphysis, neurites, neuromeres, gut epithelium |
zn-5 | IgG1,k | cell surface | subset of CNS tracts and commissures, retinal ganglion cell layer, epibranchial placodes, muscle pioneers, neural crest cells, brain (neuropil), ganglia, Rohon-Beard neurons, ventral spinal cord neurons |
zn-6 | IgG1,k | cell surface | dorsal spinal cord neurons, enteric nervous system, retinal ganglion cell layer, retinal inner plexiform layer, musculature system, brain (neuropil), retinal neural layer, retinal outer plexiform layer, peripheral nervous system, brain stem/spinal tracts and commissures |
zn-7 | IgG1 | cytoplasm | brain, gut, sensory hair cells, statoacoustic (VIII) ganglion, neural crest cells, retinal neural layer, epiphysis, posterior lateral line ganglion, posterior lateral line nerve, lateral spinal cord neurons, spinal cord |
zn-8 | IgG1,k | cell surface | subset of brain (cellular), epibranchial placodes, horizontal myoseptum, Rohon-Beard neurons, somites, trunk mesenchyme, ventral spinal cord neurons |
zn-9 | IgG1,k | cell surface | enteric nervous system, fins, hindbrain (hindbrain neuropil), fourth ventricle, retinal outer plexiform layer, peripheral nervous system, lateral spinal cord neurons, trigeminal ganglion, trigeminal nerve (cranial nerve V) |
zn-10 | IgG1 | Â | dorsal root ganglion, enteric nervous system, sensory hair cells, retinal inner plexiform layer, neurons, brain (neuropil), retinal neural layer, peripheral nervous system, YSL |
zn-11 | IgG2b | Â | dorsal root ganglion, EVL, retinal ganglion cell layer, sensory hair cells, rhombomeres, neurons, brain (neuropil), epiphysis, ganglia |
zn-12 | IgG1,k | cell surface | cranial ganglia, dorsal spinal cord neurons, enteric nervous system, dorsal fin, ventral fin, epibranchial placodes, musculature system, myotome, retinal neural layer, Rohon-Beard neurons |
zn-13 | IgM | cell surface | dorsal spinal cord neurons, enteric nervous system, sensory hair cells, musculature system, myotomes, neuropil, Rohon-Beard neurons, trigeminal ganglion, trigeminal nerve (cranial nerve V) |
zn-14 | IgM | cell surface | sensory hair cells, rhombomeres, retinal inner plexiform layer, neural crest cells, brain (neuropil and cellular), retinal outer plexiform layer, peripheral nervous system, unidentified cell types on spinal cord surface |
zn-15 | IgG1,k | cytoplasm or cell surface | brain vasculature, central nervous system, enteric nervous system, sensory hair cells, rhombomeres, retinal inner plexiform layer, neurons, brain (neuropil), retinal outer plexiform layer, epiphysis, posterior lateral line nerve, peripheral nervous system, neurites |
zna-1 | IgG1,k | Â | rhombomeres, brain (neuropil), cell somata, growth cones |
znp-1 | IgG2a,k | cell surface | rhombomeres, retinal inner plexiform layer, brain (neuropil), subset of spinal cord (cellular), ventral root, YSL |
znp-4 | IgG1 | cell surface | retinal inner plexiform layer, brain (neuropil), retinal outer plexiform layer, spinal cord |
znp-5 | IgG1 | cell surface | brain (neuropil), punctate label in medial longitudinal fasciculus, brain (neuropil), lateral spinal cord neurons, spinal cord ventral neurons |
znp-6 | IgG1,k | Â | central nervous system, EVL, gills, lens, myotomes, brain (neuropil), retinal neural layer, lateral spinal cord neurons, somites, spinal cord ventral neurons, visceral organs, YSL |
znp-7 | IgG1,k | cell surface | brain (neuropil), sensory hair cells, rhombomeres, retinal inner plexiform layer, subset of retinal neural layer and spinal cord (cellular), brain (neuropil), retinal outer plexiform layer, lateral spinal cord neurons, spinal cord ventral neurons, YSL |
zns-1 | IgG1,k | cytoplasm | central nervous system, enteric nervous system, ciliary marginal zone, rhombomere, retinal inner plexiform layer, retinal outer plexiform layer, peripheral nervous system |
zns-2 | IgG1,k | cell surface | brain (neuropil), enteric nervous system, sensory hair cells, hindbrain radial glial cell, retinal inner plexiform layer, olfactory placode, retinal outer plexiform layer, actinotrichia |
zns-5 | IgG1,k | cell surface | cranial ganglia, central nervous system, enteric nervous system, fins, sensory hair cells, rhombomeres, retinal inner plexiform layer, retinal outer plexiform layer, inner ear, peripheral nervous system |
zns-6 | IgG1,k | Â | central nervous system, ectoderm, rhombomeres, retinal inner plexiform layer, myotomes, brain (neuropil), retinal outer plexiform layer, radial glial cells |
zns-7 | IgG1,k | cytoplasm | central nervous system, DEL cells, oral epithelium, EVL, gut, horizontal myoseptum, lens, myotomes, brain (neuropil), notochord, somites, YSL |
znt-1 | IgM | cytoplasm or extracellular matrix | actinotrichia of median finfold, neural crest cells, retina, apical ectodermal ridge of pectoral fin, sensory hair cells, retinal inner plexiform layer, lens, myotome, brain (neuropil), notochord, subset of inner ear |
znu-2 | IgG1,k | Â | subset of brain (cellular), DEL cells, EVL, lens, retinal neural layer, Rohon-Beard neurons |
zpr-1 | IgG1,k | cytoplasm | retinal photoreceptor cell layer, epiphysis |
zpr-2 | IgG1,k | cytoplasm | retinal photoreceptor cell layer, epiphysis |
zpr-3 | IgG1,k | cytoplasm | retinal photoreceptor layer, photoreceptor outer segment layer |
zrf-1 | IgG1 | cytoplasm | dorsoventral diencephalic tract, gut epithelium, Muller cells, notochord, radial glial cells in rhombomeres and spinal cord, ventral roots |
zrf-2 | IgG1,k | cytoplasm | radial glial cells |
zrf-3 | IgG1,k | cell surface | unidentified structure close to meninx primitiva, radial glial cells in rhombomere , retinal inner plexiform layer, retinal outer plexiform layer, posterior lateral line nerve, radial glial cells, lateral spinal cord neurons |
zrf-4 | IgG1,k | cell surface | central nervous system, radial glial cells in rhombomeres, optic nerve (cranial nerve II), radial glial cells |
zs-3 | IgG1,k | cytoplasm | retinal neural layer |
zs-4 | IgG1,k | cytoplasm | central nervous system, DEL cells, EVL, lens, lens epithelium, musculature system, YSL |
