Position available for a highly motivated Research Assistant to join our group to investigate craniofacial
development and disease using zebrafish models and craniofacial stem cells tissue engineering approaches, in
the laboratory of Pamela C. Yelick (Pamela.yelick@tufts.edu).
Job Responsibilities:
Zebrafish mutant line breeding and screening transgenic embryos
Primary Cell Tissue Culture Techniques
Genotyping (PCR and electrophoresis)
Basic bench techniques including preparing solutions, sterile technique tissue culture.
Molecular biology techniques – DNA, RNA isolation
Histology and Immunohistochemistry – sectioning, staining and immunostaining tissues
Genome editing techniques
Single cell embryo microinjection
Imaging – whole mount, live, confocal and tissue sections
Preparing and analysis of bulk and single-cell sequencing samples
Basic laboratory tasks and ordering supplies
Ensuring that safe laboratory practices are followed, including the use and disposal of chemicals and
hazardous materials.
Experience with tissue molecular biology, imaging, tissue culture or zebrafish handling highly desired.
Education:
Bachelor's or Master's Degree in Developmental Biology, Genetics or related field.
Minimum of 1 year of wet lab bench (academic or industry) experience preferred.
Minimum two-year commitment preferred due to a substantial training effort associated with the position.
Knowledge, Skills and Abilities:
Demonstrated organizational skills and ability to pay close attention to detail.
Motivation to learn and grow in scientific expertise.
Ability to manage work with minimal supervision.
Ability to trouble-shoot technical issues.
Strong communication and presentation skills.
Ability to multi-task and work on multiple projects simultaneously.
Ability to plan and prioritize work while responding flexibly to rapidly changing priorities.
Ability to work flexible hours.
This position may require standing or sitting for long periods of time, including in a warm aquarium
environment.
Interested candidates should send their letter and CV to pamela.yelick@tufts.edu
Job Summary:
Two full-time (1.0FTE) positions for a junior level PhD post-doctoral researcher are open in the Nechiporuk lab at Oregon Health & Science University in Portland, OR. These positions will provide a mechanism for the hired individual to develop into a successful independent researcher in the fields of cell and developmental biology. The Nechiporuk lab uses zebrafish as a model system to study mechanisms of the peripheral sensory system development and function. The hired individual will use variety of modern approaches, including transgenesis, CRISPR-based genome editing, single-cell RNA sequencing (scRNA-seq), and live imaging, to dissect intracellular processes that drive axon growth, neurotrophin signaling, and collective cell migration during development and disease. Successful candidates are expected to have a published record of accomplishment appropriate to level, and good written and oral communication skills.
Experience in model organism biology, cloning, molecular biology of DNA/RNA/protein, microscopy, CRISPR, complex genetics, bioinformatics is a plus.
Salary and Benefits:
The hiring salary for this position is set according to NIH guidelines.
Benefits include medical and dental insurance as well as retirement benefits.
Minimum Qualifications:
PhD in life sciences such as genetics, anatomy, biology, biochemistry, or related field
Interested individuals should email their CV, short statement of research interests, and the names and contact information for three references to Alex Nechiporuk (nechipor@ohsu.edu)
The Clemson University Center for Human Genetics and the Greenwood Genetic Center have been awarded a five year Phase I COBRE in Human Genetics grant to understand the genetic, genomic, and epigenetic mechanisms by which molecular genetic variation affects transcriptional and other molecular networks in health and disease. The Flanagan-Steet lab at the GGC is seeking a post-doctoral researcher to investigate mechanisms driving disease in rare genetic disorders. The successful candidate will combine systems-based approaches with studies in cell and animal systems to investigate molecular and cellular disease mechanisms. Zebrafish experience and expertise with microscopy are helpful but not required. Salary will be commensurate with credentials and experience.
Enquiries should be addressed to Dr. Heather Flanagan-Steet, COBRE Project Leader and Director of Functional Studies, Greenwood Genetic Center, 113 Gregor Mendel Circle, Greenwood, SC 29646 (heatherfs@ggc.org). Applications must include a cover letter explaining the qualifications for this position and the names of three references. The position is available immediately. The Greenwood Genetic Center is an equal opportunity employer.
Email: Dr. Heather Flanagan-Steet, heatherfs@ggc.org
Email: careers@ggc.org
Fax: (864) 388-1062
The Greenwood Genetic Center is an Equal Opportunity Employer
A grant-supported postdoctoral position aimed at defining pathogenic mechanisms of pediatric disease is available immediately in the Steet lab within the JC Self Research Institute of the Greenwood Genetic Center (GGC). The GGC is a non-profit clinical, diagnostic and research center whose mission is to advance diagnoses and treatment for children with rare genetic diseases. The Research Division is equipped with state-of-the-art technology and equipment, including a new aquaculture facility. This position offers a unique opportunity to engage directly with clinical and molecular geneticists to better understand the genetic basis and molecular pathogenesis of inherited disorders.
We are seeking a highly motivated individual to address questions regarding disease pathogenesis. Zebrafish experience and expertise with microscopy is helpful but not required. The successful candidate will use a combination of cell and animal systems in a highly collaborative environment to investigate disease mechanisms at a molecular and cellular level. Applicants are expected to have a strong record of first author publications from their Ph.D. work, experience with molecular and cell biological techniques and skill in both written and verbal communication. Interested applicants should send their cover letter, resume and list of professional references to Dr. Heather Flanagan-Steet.
Email: Dr. Heather Flanagan-Steet, heatherfs@ggc.org
Email: careers@ggc.org
Fax: (864) 388-1062
The Greenwood Genetic Center is an Equal Opportunity Employer
The Department of Pathobiology (vetmed.illinois.edu/path) at the College of Veterinary Medicine, University of Illinois Urbana-Champaign is accepting applications for a Visiting Veterinary Research Specialist. This is a full-time, 12-month, non-tenure track position. Salary is commensurate with experience and qualifications. This appointment may become non-visiting at a later date.
The University of Illinois is an Equal Opportunity, Affirmative Action employer that recruits and hires qualified candidates without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, disability or veteran status. For more information, visit http://go.illinois.edu/EEO.
The successful candidate will support animal and molecular research in Dr. Christopher Gaulke’s Laboratory. The candidate will perform Zebrafish husbandry and assist in original microbiome research under the guidance of the principal investigator. Other duties include recording, analyzing, organizing and presenting experimental results; training new staff and students; preparing and writing manuscripts in collaboration with the principal investigator; and preforms day to day operations including inventory, ordering, regulatory and safety compliance. Minimum education requirements include a Bachelor of Science Degree in Microbiology, Molecular Biology, Fisheries or a related field. Candidate must have at least six months laboratory experience, experience preforming standard laboratory techniques (e.g., PCR, aseptic technique, etc.), foundational knowledge of statistics, and experience working with model organisms to be considered for this position. Preferred experience includes at least two years of aquaculture experience. Experience working with zebrafish and mice, practical knowledge of plumbing, command line proficiency, familiarity with one or more scripting languages (e.g., Perl, python, etc.), proficiency in R, experience with immunohistochemistry and confocal imaging, proficient in molecular cloning, experience analyzing microbiome data, experience analyzing large multiomic datasets.
For consideration, create a candidate profile at jobs.illinois.edu and upload application materials, including a cover letter, curriculum vitae or resume, and the names and email contact information for at least three professional references by April 7, 2021. All requested information must be submitted prior to consideration. Applicants may be interviewed prior to the closing date; however, no hiring decisions will be made until after April 7, 2021.
For further information contact Karen Nichols at klp68@illinois.edu or (217) 300-8343.
The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer. The University of Illinois System requires candidates selected for hire to disclose any documented finding of sexual misconduct or sexual harassment and to authorize inquiries to current and former employers regarding findings of sexual misconduct or sexual harassment. For more information, visit Policy on Consideration of Sexual Misconduct in Prior Employment. As a qualifying federal contractor, the University of Illinois System uses E-Verify to verify employment eligibility.
The National Institutes of Health is a world-leading biomedical research campus located in Bethesda, Maryland made up of 27 individual, mission-centered institutes. It also serves as the main funding agency for biomedical research throughout the United States. The National Human Genome Research Institute (https://www.genome.gov/about-nhgri) has a specific focus on understanding how the genome functions and how alterations in the genome impact human health.
Postdoctoral Opportunity:
The Burgess lab (https://www.genome.gov/staff/Shawn-Burgess-PhD ) is seeking a Postdoctoral Fellow for a fully funded position (international applications are possible). The lab has broad interests in identifying the genes and regulatory networks necessary for hearing and/or other tissue regeneration in zebrafish. There are also opportunities for developing new model systems, computational projects including genome assembly, and technology development to leverage zebrafish in high-throughput screening approaches such as drug screening or CRISPR/Cas. There are many projects available and preference is given for ideas generated by the applicant.
Minimum Qualifications are a PhD, MD or equivalent degree, specific relevant training in the topics is not essential.
If interested please send:
a cover letter describing relevant qualifications, questions you might be interested in pursuing, and specific career goals.
a current CV
names and contact information of three references
Send inquiries or application materials in an email to burgess@mail.nih.gov
Laboratory Technician:
Link to UL career site for applications: http://emea3.mrted.ly/2nvdn
The University of Luxembourg aspires to be one of Europe’s most highly regarded universities with a distinctly international and interdisciplinary character. It fosters the cross-fertilisation of research and teaching, is relevant to its country, is known worldwide for its research and teaching in targeted areas, and is establishing itself as an innovative model for contemporary European Higher Education. The University`s core asset is its well-connected world-class academic staff which will attract the most motivated, talented and creative students and young researchers who will learn to enjoy taking up challenges and develop into visionary thinkers able to shape society.
Within the University, the Luxembourg Centre for Systems Biomedicine (LCSB) is a highly interdisciplinary research centre (IC), integrating experimental biology and computational biology approaches in order to develop the foundation of a future predictive, preventive and personalized medicine.
Area: the applicant will be a member of the Enzymology & Metabolism Research Group headed by Assist. Prof. Dr. Carole Linster at the LCSB. The Group’s research currently focuses on elucidating the physiological functions of more recently identified metabolic enzymes and on developing strategies for accelerating enzyme function discovery. A second central topic is the generation of disease models for selected inborn errors of metabolism to study underlying disease mechanisms and/or investigate treatment options. The group develops advanced phenotyping techniques, including metabolomics, to address related research questions and uses the budding yeast, zebrafish, and mammalian cell culture, including iPSC-derived cells, as main model systems.
Your Role
We seek a highly skilled and motivated laboratory technician to work on an EU-funded research project within an international consortium that focuses on two inherited disorders of lysine metabolism causing debilitating and progressive neurological symptoms in young children. In brief, the Luxembourg contribution to the project includes the generation and deep characterization of several in vivo disease models using zebrafish to ultimately evaluate a number of innovative strategies to improve early diagnosis and early implementation of personalized therapies to enhance long-term health outcomes and patients’ quality of life.
More specifically your tasks will include:
Contribute to the generation of stable mutant lines (via CRISPR/Cas9 gene editing) in zebrafish
Perform molecular biology experiments, such as PCR-based genotyping, cloning
Contribute to the performance of experiments using zebrafish embryos/larvae, e.g. microinjections, behavioral tests, open field recordings, immunohistochemistry
Contribute to the performance of metabolomics experiments, such as extractions for LC-MS metabolite analysis
Perform supporting tasks for the maintenance and breeding of zebrafish according to guidelines and instructions
Modern data management and precise project documentation
Contribute to meeting preparation and reporting within the international consortium
Capacity to work in a collaborative environment among different service-based platforms, e.g. aquatic, metabolomics, microscopy
Ordering consumables and maintaining stocks
Maintenance of a clean laboratory setting
Participation in group meetings and monthly technical meetings
Follow and comply with safety regulations and legislation
Your Profile
A technical, Bachelor or Master degree in biochemistry, molecular biology, genetics, neurosciences, biotechnology or another similar life science related discipline
Experience with molecular biology techniques is essential
Experience with working with animals is strongly preferred (animal laboratory science certificate Function A according to the European Directive 2010/63/EU is required). Previous experience with aquaculture techniques and/or with zebrafish will be considered as an advantage
Excellent organizational and management skills
Solid computational skills
Good working knowledge of English is required. Knowledge of either German, French, or Luxemburgish will be considered as an advantage
We offer
A key role in cutting-edge research projects related to modeling human diseases
Opportunity to work in an exciting international, interdisciplinary research environment
Opportunity to participate in the development of the group’s research
Opportunity for professional trainings
In Short
Contract Type: Fixed Term Contract 24 Month
Work Hours: Full Time 40.0 Hours per Week
Location: Belval
Foreseen starting date: May 2021
Job Reference: UOL04030
Further Information
Applications (in English) should contain the following documents:
A detailed Curriculum vitae
A motivation letter that includes a brief description of previous research experience
Copies of the relevant diplomas
A scan of your animal laboratory science certificate (Function A required)
Name and contact details of at least two referees
Early application is highly encouraged, as the applications will be processed upon reception. Please apply ONLINE (http://emea3.mrted.ly/2nvdn) formally through the HR system. Applications by email will not be considered.
The University of Luxembourg embraces inclusion and diversity as key values. We are fully committed to removing any discriminatory barrier related to gender, and not only, in recruitment and career progression of our staff.
Here’s what awaits you at the University
Multilingual and international character. Modern institution with a personal atmosphere. Staff coming from 90 countries. Member of the “University of the Greater Region” (UniGR).
*A modern and dynamic university. *High-quality equipment. WiFi on campus. Close ties to the business world and to the Luxembourg labour market. A unique urban site with excellent infrastructure.
A partner for society and industry. Cooperation with European institutions, innovative companies, the Financial Centre and with numerous non-academic partners such as ministries, local governments, associations, NGOs …
Addresses, maps & routes to the various sites of the University
Research & Development Specialist in Systems Biomedicine with focus on disease modeling:
Link to UL career site for applications: http://emea3.mrted.ly/2nvki
The University of Luxembourg aspires to be one of Europe’s most highly regarded universities with a distinctly international and interdisciplinary character. It fosters the cross-fertilisation of research and teaching, is relevant to its country, is known worldwide for its research and teaching in targeted areas, and is establishing itself as an innovative model for contemporary European Higher Education. The University`s core asset is its well-connected world-class academic staff which will attract the most motivated, talented and creative students and young researchers who will learn to enjoy taking up challenges and develop into visionary thinkers able to shape society.
Within the University, the Luxembourg Centre for Systems Biomedicine (LCSB) is a highly interdisciplinary research centre (IC), integrating experimental biology and computational biology approaches in order to develop the foundation of a future predictive, preventive and personalized medicine.
Area: the applicant will be a member of the Enzymology & Metabolism Research Group headed by Assist. Prof. Dr. Carole Linster at the LCSB. The Group’s research currently focuses on elucidating the physiological functions of more recently identified metabolic enzymes and on developing strategies for accelerating enzyme function discovery. A second central topic is the generation of disease models for selected inborn errors of metabolism to study underlying disease mechanisms and/or investigate treatment options. The group develops advanced phenotyping techniques, including metabolomics, to address related research questions and uses the budding yeast, zebrafish, and mammalian cell culture, including iPSC-derived cells, as main model systems.
Your Role
We seek a highly skilled and motivated researcher to develop an EU-funded project within an international consortium that focuses on two rare disorders of lysine metabolism causing debilitating and progressive neurological symptoms in young children. In brief, the Luxembourg contribution to the project includes the generation and deep characterization of several in vivo disease models using zebrafish to ultimately evaluate a number of innovative strategies to improve early diagnosis and early implementation of personalized therapies to enhance long-term health outcomes and patients’ quality of life.
More specifically your tasks will include:
Generation of stable mutant lines (via CRISPR/Cas9 gene editing) in zebrafish
Performance of experiments using zebrafish embryos/larvae, e.g. microinjections, behavioral tests, open field recordings, immunohistochemistry
Performance of molecular biology experiments, such as PCR-based genotyping, cloning
Performance of metabolomics experiments, such as extractions, LC-MS metabolite analysis
Modern data management and precise project documentation
Active meeting participation and reporting within the international consortium
Capacity to work in a collaborative environment among different service-based platforms, e.g. aquatic, metabolomics, microscopy
Ordering consumables and maintaining stocks
Maintenance of a clean laboratory setting
Active participation in group meetings
Your Profile
A Master’s degree in biochemistry, molecular biology, genetics, neurosciences, biotechnology or another similar life science related discipline
Previous work in the field of metabolic biochemistry and/or neurosciences will be considered as an advantage
Experience with molecular biology techniques is essential
Experience with working with animals is essential (animal laboratory science certificate Function A or B according to the European Directive 2010/63/EU is required). Previous experience with aquaculture techniques and/or with zebrafish will be considered as an advantage
Excellent organizational and management skills
Excellent communication skills
Strong computational skills
Fluency in oral and written English is essential
We offer
A key role in cutting-edge research projects related to modeling human diseases
Opportunity to work in an exciting international, interdisciplinary research environment
Opportunity to participate in the development of the group’s research
Opportunity for professional trainings
In Short
Contract Type: Fixed Term Contract 24 Month with possible extension
Work Hours: Full Time 40.0 Hours per Week
Location: Belval
Foreseen starting date: May 2021
Job Reference: UOL04035
Further Information
Applications (in English) should contain the following documents:
A detailed Curriculum vitae
A motivation letter that includes a brief description of previous research experience and future interests
Copy of Master degree diploma
A scan of your animal laboratory science certificate (Function A or B required)
Name and contact details of at least two referees
Early application is highly encouraged, as the applications will be processed upon reception. Please apply ONLINE (http://emea3.mrted.ly/2nvki) formally through the HR system. Applications by email will not be considered.
The University of Luxembourg embraces inclusion and diversity as key values. We are fully committed to removing any discriminatory barrier related to gender, and not only, in recruitment and career progression of our staff.
Here’s what awaits you at the University
Multilingual and international character. Modern institution with a personal atmosphere. Staff coming from 90 countries. Member of the “University of the Greater Region” (UniGR).
*A modern and dynamic university. *High-quality equipment. WiFi on campus. Close ties to the business world and to the Luxembourg labour market. A unique urban site with excellent infrastructure.
A partner for society and industry. Cooperation with European institutions, innovative companies, the Financial Centre and with numerous non-academic partners such as ministries, local governments, associations, NGOs …
Addresses, maps & routes to the various sites of the University
The University of Mississippi Medical Center Cancer Institute and Department of Neurosurgery seek a staff scientist / laboratory head to lead a research effort in brain tumor biology.
We are looking for a highly motivated, independent, and career-oriented research scientist with an excellent publication record.
Preferred Qualifications
· PhD in cancer biology, developmental biology, cell biology, neuroscience or related field
· hands on experience conducting cellular and animal experiments using zebrafish and/or mouse xenografts.
Excellent oral and written communication skills are necessary. Strong cellular and molecular biology and animal (zebrafish or mouse) model training are required. Candidates must have first-author publications and a solid publication record.
Research experience in two or more of the following areas are desirable:
· Zebrafish cancer xenografts and assays of metastasis
· Zebrafish development
· Mouse cancer xenografts and assays of metastasis
· Cell metabolism
· Cancer cell biology
· In vivo imaging
To apply please include CV and at least three references.
We are seeking a motivated postdoctoral scholar to join Dr. Genevieve Kendall’s lab at Nationwide Children’s Hospital and The Ohio State University. Our team is focused on developing basic and translational models of pediatric muscle cancer, which are devastating and aggressive solid tumors with limited therapeutic options. We use cell culture and transgenic zebrafish systems to understand the underlying biology of the disease and then apply that knowledge to identify therapeutic targets.
With guidance from Dr. Kendall, you will be responsible for leading an independent project that addresses our shared interests and aligns with your career goals. Your responsibilities will include performing experiments, determining the next steps, developing and implementing new and existing protocols, analyzing and presenting your results and conclusions, generating figures, and writing and publishing manuscripts. A background in cancer biology and/or genomics would be beneficial. The selected candidate must be professional, self-motivated, and dedicated, with strong communication and interpersonal skill sets that fit our team. You will have many opportunities to learn new skill sets, present your work, develop professionally, and contribute to identifying new therapies for pediatric cancers in a dynamic and positive environment.
Visit our lab website for more information about our research and team: https://www.nationwidechildrens.org/research/areas-of-research/center-for-childhood-cancer-and-blood-diseases/kendall-lab'
**Please include typed cover letter**
Schedule: Full time, Benefits Eligible
Location:
Abigail Wexner Research Institute
700 Children’s Drive
Columbus, OH 43205
Why Nationwide Children’s Hospital
The moment you walk through our doors, you can feel it. When you meet one of our patient families, you believe it. And when you talk with anyone who works here, you want to be part of it, too. Welcome to Nationwide Children’s Hospital, where Passion Meets Purpose.
Here, Everyone Matters. We’re 12,000 strong. And it takes every single one of us to improve the lives of the kids we care for, and the kids from around the world we’ll never even meet. Kids who are living healthier, fuller lives because of the knowledge we share. We know it takes a Collaborative Culture to deliver on our promise to provide the very best, innovative care and to foster new discoveries, made possible by the most groundbreaking research. Anywhere.
Ask anyone with a Nationwide Children’s badge what they do for a living. They’ll tell you it’s More Than a Job. It’s a calling. It’s a chance to use and grow your talent to make an impact that truly matters. Because here, we exist simply to help children everywhere.
Nationwide Children’s Hospital. A Place to Be Proud
Responsibilities
Designing and conducting laboratory-based experiments in cell culture and/or transgenic zebrafish systems
Collecting experimental data and maintaining an updated lab notebook
Critically evaluating and interpreting data and determining next steps
Mentoring and training staff and graduate students
Generating figures
Writing and publishing scientific material, including manuscripts
Presenting research at regional, national, and international conferences
Contributing to grant submissions for external funding
Collaborating with other researchers at NCH/OSU, nationally, and internationally
KNOWLEDGE, SKILLS AND ABILITIES REQUIRED:
To fulfill this role successfully, you must possess these qualifications and experience:
MD, Ph.D., Pharm.D., or equivalent degree.
Desire to pursue a career in biomedical research
Technical skills related to cell culture and/or working with transgenic zebrafish, genomics data analysis, and a desire to learn new skills
Ability to work independently on laboratory projects
Proficiency in scientific writing
Excellent communication skills
Ability to train and mentor laboratory staff and graduate students
Minimum Physical Requirement:
Ability to lift objects weighing up to 20 lbs (Occasionally)
Sitting (Frequently)
Talking on the telephone and in person
Typing on a keyboard (Frequently)
Walking (Frequently)
The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the individual so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct, and control the work of employees under his/her supervision.
EOE/M/F/Disability/Vet
Our Company
InVivo Biosystems (formerly NemaMetrix Inc.) is a fast-paced, biotechnology company whose mission is to enable scientists and researchers around the world to better understand human health by offering a more affordable and accessible platform. We develop solutions for the genetic modification of C. elegans and Zebrafish to act as human proxies for disease research and drug discovery and help our customers reveal new insights into the true nature of genetic mutations. InVivo Biosystems is headquartered in Eugene, Oregon with a satellite office located in Salt Lake City, Utah.
Role: Laboratory Tech III/IV - Zebrafish
Location: Eugene, OR
Overview:
Working as part of our Zebrafish Gene Editing team, this role is designed to deliver genome edited animals for both internal and external clients. Using established workflows, this hands-on bench role is responsible for the execution and delivery of daily/weekly project tasks and meeting delivery milestones. Additionally, this role will be expected to identify areas of improvement, apply creative problem solving to overcome project roadblocks, and serve as a technical resource for advanced gene editing methodologies.
What You’ll be Doing:
· Designing repair templates and constructs for CRISPR and Tol2 mediated gene editing in Zebrafish.
· Using standard and advanced molecular biology techniques for detecting precise edits in vivo.
· Screening and propagation of de novo genome edited Zebrafish.
· Troubleshooting innovative technologies to validate design and detect editing events.
· Working collaboratively within a team to meet commercial timelines, with the ability to deliver on multiple projects simultaneously.
· Providing execution and adherence to SOPs, detailed project tracking and documentation.
· Supporting reagent, equipment and maintenance requests and other related activities.
· Contributing to continuous process improvement to optimize timelines in the engineering pipeline.
What You Bring to the Table: InVivo Biosystems is looking for individuals that possess a positive can-do attitude, are motivated by doing a job well, are adaptable and flexible, have a continuous improvement mindset and will thrive in a fast-paced collaborative and diverse environment. In addition, successful candidates will have the following:
Required Qualifications
· BS in molecular biology, bioengineering or similar life sciences field and 4-7 years of related experience required, MS degree in similar fields with at least 2 years relevant experience preferred.
· Hands on experience with CRISPR genome editing methodologies (KO and HDR).
· Hands on experience with Tol2 transposon based transgenesis.
· Experience working with genome assemblies such as ensemble or NCBI.
· Experience with design of plasmids using vector building software such as Benchling, Ape or equivalent.
· Hands on experience with standard RE cloning methodology and diagnostic techniques.
· Proficient in standard molecular biology techniques including PCR, primer design, DNA Sequence Analysis.
· In-vitro transcription and RNA purification.
Preferred Qualifications
· Previous experience with microinjection.
· Ability to design and interpret High Resolution Melting (HRM) analyses.
· Functional knowledge of advanced homology based cloning methods such as Gibson Assembly, Golden Gate or Infusion.
· Preference will be given to applicants with previous Zebrafish experience.
TO APPLY: Go to https://invivobiosystems.com/careers/ and apply to this specific position. Note: job level will be dependent of the successful candidate’s combination of relevant education and experience.
How We Take Care of Our Humans
In addition to our friendly and collegial staff, we offer a benefits package that includes:
· Medical, Dental, and Vision insurance
· Basic and Supplemental Life and AD&D Insurance
· 401k Plan
· Paid Time Off
· Paid Holidays
· Flexible work schedules
· Employee Events – including Summer BBQs, community service, and holiday-related events (virtually and in-person when we are able)
· COVID-19 precautions include remote (Zoom) interviewing, remote meetings, office and lab safety protocols as well as regular environmental testing in our facilities.
Our Commitment to Diversity, Equity & Inclusion
Inclusion, it’s in our DNA. At InVivo Biosystems, we’re passionate about creating an inclusive workplace that encourages, supports, and celebrates diversity. These are not just words for us – we actively seek to create an environment that reflects the global community we serve and where everyone feels empowered to bring their full, authentic selves to work.
We are scientists, researchers, business people, and barrier breakers - we are unique individuals. We believe diversity, equity, and inclusion drive innovation. Together, we spark the insights that create a better, healthier world.
Equal Opportunity Statement
InVivo Biosystems is an equal opportunity/affirmative action employer. We recruit, employ, train, compensate and promote regardless of race, religion, color, national origin, sex, disability, age, sexual orientation, gender identity, veteran status, and any other protected category as required by applicable law.
Our Company
InVivo Biosystems (formerly NemaMetrix Inc.) is a fast-paced, biotechnology company whose mission is to enable scientists and researchers around the world to better understand human health by offering a more affordable and accessible platform. We develop solutions for the genetic modification of C. elegans and Zebrafish to act as human proxies for disease research and drug discovery and help our customers reveal new insights into the true nature of genetic mutations. InVivo Biosystems is headquartered in Eugene, Oregon with a satellite office located in Salt Lake City, Utah.
Role: Scientist I – Zebrafish
Location: Eugene, OR
Overview:
Working as a critical part of our Zebrafish Gene Editing team, this role is designed to deliver genome edited animals for both internal and external clients. Using established workflows, this hands-on bench role is responsible for the execution and delivery of daily/weekly project tasks and meeting delivery milestones. Additionally, this role will be expected to identify areas of improvement, apply creative problem solving to overcome project roadblocks, and serve as a technical expert for advanced gene editing methodologies.
What You’ll be Doing:
· Designing repair templates and constructs for CRISPR and Tol2 mediated gene editing in Zebrafish.
· Using standard and advanced molecular biology techniques for detecting precise edits in vivo.
· Screening and propagation of de novo genome edited Zebrafish.
· Identifying and troubleshooting innovative technologies to validate design and detect editing events.
· Working independently and within a team to meet commercial timelines, with the ability to deliver on multiple projects simultaneously.
· Providing execution and adherence to SOPs, detailed project tracking and documentation.
· Supporting reagent, equipment and maintenance requests and other related activities.
· Contributing to continuous process improvement to optimize timelines in the engineering pipeline.
What You Bring to the Table: InVivo Biosystems is looking for individuals that possess a positive can-do attitude, are motivated by doing a job well, are adaptable and flexible, have a continuous improvement mindset and will thrive in a fast-paced collaborative and diverse environment. In addition, successful candidates will have the following:
Required Qualifications
· Masters degree in molecular biology, bioengineering or similar life sciences field and 2-4 years of related experience or a bachelor’s degree in similar fields with at least 4 years relevant experience.
· Hands on experience with CRISPR genome editing methodologies (KO and HDR).
· Hands on experience with Tol2 transposon based transgenesis.
· Experience working with genome assemblies such as ensemble or NCBI.
· Experience with design of plasmids using vector building software such as Benchling, Ape or equivalent.
· Hands on experience with standard RE cloning methodology and diagnostic techniques.
· Proficient in standard molecular biology techniques including PCR, primer design, DNA Sequence Analysis.
· In-vitro transcription and RNA purification.
Preferred Qualifications
· Previous experience with microinjection.
· Ability to design and interpret High Resolution Melting (HRM) analyses.
· Functional knowledge of advanced homology based cloning methods such as Gibson Assembly, Golden Gate or Infusion.
· Preference will be given to applicants with previous Zebrafish experience.
TO APPLY: Go to https://invivobiosystems.com/careers/ and apply to this specific position.
How We Take Care of Our Humans
In addition to our friendly and collegial staff, we offer a benefits package that includes:
· Medical, Dental, and Vision insurance
· Basic and Supplemental Life and AD&D Insurance
· 401k Plan
· Paid Time Off
· Paid Holidays
· Flexible work schedules
· Employee Events – including Summer BBQs, community service, and holiday-related events (virtually and in-person when we are able)
· COVID-19 precautions include remote (Zoom) interviewing, remote meetings, office and lab safety protocols as well as regular environmental testing in our facilities.
Our Commitment to Diversity, Equity & Inclusion
Inclusion, it’s in our DNA. At InVivo Biosystems, we’re passionate about creating an inclusive workplace that encourages, supports, and celebrates diversity. These are not just words for us – we actively seek to create an environment that reflects the global community we serve and where everyone feels empowered to bring their full, authentic selves to work.
We are scientists, researchers, business people, and barrier breakers - we are unique individuals. We believe diversity, equity, and inclusion drive innovation. Together, we spark the insights that create a better, healthier world.
Equal Opportunity Statement
InVivo Biosystems is an equal opportunity/affirmative action employer. We recruit, employ, train, compensate and promote regardless of race, religion, color, national origin, sex, disability, age, sexual orientation, gender identity, veteran status, and any other protected category as required by applicable law.
The MDI Biological Laboratory is a rapidly growing, independent, non-profit biomedical research institution. Its mission is to improve human health and well-being through basic research, education, and development ventures that transform discoveries into cures.
In 2013, the MDI Biological Laboratory was recognized by the National Institutes of Health as a Center of Biomedical Research Excellence (COBRE) for its innovative research program in regenerative and aging biology and medicine. The Institution’s expert, interdisciplinary faculty utilizes multiple genetically tractable model organisms and diverse cell systems to define mechanisms of tissue repair and regeneration and characterize changes that occur in cells and tissues with disease and aging.
Job Summary
We are currently accepting applications for a Post-doctoral research fellow in the Madelaine laboratory (email rmadelaine@mdibl.org) with an anticipated start date in September, 2021. Our research focuses on understanding the mechanisms of neural stem cell activation and neuronal regeneration. We primarily use the zebrafish as a model organism to study the molecular and cellular mechanisms of retinal and olfactory neurons regeneration.
Our laboratory in the Kathryn W. Davis Center for Regenerative Biology and Aging employs a range of imaging and molecular technologies including confocal microscopy, live imaging, transgenesis, CRISPR-genome engineering and single cell RNA sequencing to study the biological mechanisms underlying neural stem cell reprogramming in vivo. We are seeking a highly motivated postdoctoral researcher with a strong background in developmental biology and/or physiology, or related field. The successful candidate will be expected to conduct research using cellular and molecular biological techniques to characterize the mechanisms regulating retinal and olfactory neural stem cells function during neuronal regeneration.
The collaborative research environment at the MDIBL offers an excellent opportunity for career development. Funding is available for 3 years, with the expectation that the candidate will work with Dr. Madelaine to apply for fellowships during this time. The MDIBL offers a benefits package and competitive salary.
Minimum Qualifications
Ph.D. in a biological science or other relevant area and prior publication of original research articles in peer-reviewed journals.
Preferred Qualifications
Experience with basic cell and molecular biology techniques.
Experience with single cell RNA sequencing experiments and biological datasets analysis.
Applicants should have the ability to interpret data, summarize findings, and work independently and collaboratively to synthesize new ideas to develop their project(s).
Application process
Please include:
a letter of intent outlining special interest in the position, overall related qualifications and experience and career goals.
curriculum vitae
the names and contact information of three professional references
Interested applicants should apply at www.mdibl.org/careers.
Contact: pascale.bomont@inserm.fr
Closing date: 31 March 2021
In the context of an AFM-Telethon funded “strategic project” (2021-2024): « Therapy for Giant Axonal Neuropathy», the Bomont laboratory, and the partner Puccio are seeking highly motivated candidates in the development of therapy for neuromuscular diseases.
Science. Bomont laboratory has a long-lasting expertise in the fatal neuromuscular disease called Giant Axonal Neuropathy (GAN) (1). The team identified the defective gene (2), which encodes for the Gigaxonin-E3 ligase and modelized the disease in mouse and zebrafish, to unravel pivotal roles of Gigaxonin in cytoskeleton architecture (3), autophagy maturation (4) and motoneuron specification (5) . With robust animal models in hands, and the identification of therapeutic candidates, the team aims to conduct a dual preclinical study for GAN: using pharmacological and gene therapy approaches. This translational project will benefit from the expertise of the Puccio team, which succeeded in developing gene therapy programs for Friedreich Ataxia (6-8) .
Refs: 1. Lescouzères and Bomont. Front Physiol. (2020); 2. Bomont et al., Nat Genet (2000); 3. Mahammad et al., J Clin Invest (2013)-leading group: RD. Goldman; 4. Scrivo at al., Nat Commun (2019); 5. Arribat, Mysiak et al., J Clin Invest (2019); 6. Perdomini et al., Nat Med. (2014); Piguet et al., Mol Ther. (2018); Belbellaa et al., Hum Mol Genet. (2019).
Profiles. 3-year positions for 1 postdoctoral researcher (Bomont) and 2 engineers (Bomont & Puccio); will work in close collaboration with other members of the teams. Mandatory for all: curious, enthusiastic & team player; prior experience with mice. Postdoc expertise: neurobiology of the mouse with a strong knowledge in histopathology. Engineer 1 expertise: at the interface between zebrafish & mouse; zebrafish experience is an asset but not mandatory; skills required in histopathology. Engineer 2 expertise: skills in molecular biology techniques; immunofluorescence and histopathology are assets but not required. French speaking is NOT a requirement.
When/how to apply. Starting date ideally in mid 2021, but due to the pandemic, we will accommodate the selected candidates. Deadline for submission is fixed to 31th March 2021. Please send your application in a SINGLE 3 page-long pdf, including you CV, summary of your previous research experiences, and contact information of 2-3 references to:
Where. Bomont and Puccio teams are both at the NeuroMyoGene institute, located in the Rockefeller campus, in the highly dynamic and international city of Lyon, France. Lyon is considered as one of the most attractive metropoles in Europe, with a strong signature in biological science, architecture, & gastronomy (which we hope to enjoy soon again!)
Link to Bomont webpage: https://www.inmg.fr/bomont/?lang=en
Postdoctoral Position, Heart and Vascular Development and Regeneration, University of California, San Diego
A fully funded postdoctoral position is available at the University of California, San Diego to study heart and vascular development and regeneration in the zebrafish, mouse and human pluripotent stem cells. Our laboratory uses cellular, molecular, genetic/epigenetic, single cell genomic and transgenic approaches to study the specification, patterning, and differentiation of cardiovascular progenitor cells that give rise to the heart and the vascular system. We are most interested in translating these findings for therapies and early diagnosis of cardiovascular diseases, including coronary artery disease, stroke, heart failure and cardiac arrhythmias.
Please refer to our website (http://cardiology.ucsd.edu/research/labs/chi-lab/Pages/default.aspx) and recent publications for further information:
Ren et al., Dev Cell. 2019. 23;50(6):729-743. PMID: 31402282; Galvez-Santisteban et al., Elife. 2019. 25;8. PMID: 31237233; Han et al., Nature. 2016, 30;534(7609):700-4. PMID: 27357797
Disciplinary specialty of research: Development, Stem Cell Biology, Regeneration, Genomics, Epigenetics, Bioinformatics, Single cell genomics, Spatial transcriptomics
Skills and Knowledge Required: Experience with stem cells, genomics, epigenomics, bioinformatics, developmental biology, and/or live imaging is preferred. Ph.D. or M.D. is required.
Salary: Salary commensurate with NIH pay scale up to 3-5 years with excellent benefits
Procedure to Apply: Outstanding and highly motivated candidates with a Ph.D. or M.D. should apply by email to Dr. Neil Chi (nchi@ucsd.edu) and include a CV/resume.--