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FIXATIVES
FIXATIVES
Fix buffer:
4% sucrose
0.15 mM CaCl2
0.1 M PO4 pH 7.3.
For general fixation:
1.5% glutaraldehyde, 0.5% paraformaldehyde in fix buffer.
For antibody labeling:
4% paraformaldehyde in fix buffer.
*FREEZING MEDIUM
WITHOUT Methanol*
Ginsburg Fish Ringers 10 ml (room temp.)
Powdered Skim Milk 1.5 gm
WITH Methanol
Ginsburg Fish Ringers 9 ml (room temp.)
Methanol 1 ml
Powdered Skim Milk 1.5 g
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