This page represents a portion of the MGH/CVRC Zebrafish Server content, originally hosted at http://zebrafish.mgh.harvard.edu. |
This is the latest version of the map, totalling 2000 markers and presented by Nobuyoshi Shimoda at the 1998 Cold Spring Harbor Zebrafish meeting, is available as follows:
61 markers from the previous map (Knapik et al., 1998) were excluded from this version of the map; a list is available
Good luck mapping!
A complete table for all of the primer sequences is rather cumbersome for viewing as a single document in a web browser. For that reason, you may either view them in tabular format for each of the linkage groups, or you may download a file containing all of the data (an Excel spreadsheet [483K], you might want to get a copy of the legend too).
The figures for linkage groups 1, 2, 8, 9, 10, 11, 12, 15, 16, 17, 18, 19 and 21have been 'flipped' to match the orientation used by Postlethwait et al. (Nature Gentics 18 p. 345, 1998). Linkage group 4 has more serious differences between our data and Postlethwait's, but the discrepancy has not been resolved so the figure has not been altered.
PROFILE:
Presoak: 94 degrees C for 5.0 minutes
Denaturation: 94 degrees C for 1.0 minute
Annealing: 58 degrees C for 1.0 minute
Polymerization: 72 degrees C for 1.5 minute
PCR Cycles: 27
Thermal Cycler: MJ Research PTC-100
BUFFER:
MgCL2: 1.5 mM
KCl: 50 mM
Tris-HCl: 10 mM, pH: 8.3
REAGENTS:
Template: 10 ng
Primer: 375 nM each
dNTPs: 200 uM each
Taq Polymerase: 0.034 units/ul
Total Vol: 10 ul